Copy Link Print Information

PAI1 Antibody (YA1524)

(Synonyms: SERPINE1; PAI1; PLANH1; Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1)
Customer Review

Based on 1 Customer Validation

PAI1 Antibody (YA1524) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAI1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PAI1 Antibody (YA1524)
  • PAI1 Antibody (YA1524) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PAI1 Antibody (YA1524) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAI1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database
Immunogen

Recombinant protein of PAI1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103884

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PAI1 Antibody (YA1524)
    Western blot analysis of extracts from MCF-7(lane 2(20μg), HepG2(lane 3(20μg), HuH-7(lane 4(20μg) and NIH-3T3(lane 5(20μg) using PAI1 Antibody (HY-P81779). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PAI-1 (Plasminogen activator inhibitor-1, also known as SERPINE1) is a member of serine protease inhibitor (SERPIN) family that acts as the primary inhibitor of two main mammalian plasminogen activators, urinary-type (uPA) and tissue-type (tPA) . As the main negative regulator of plasminogen activation, PAI-1 is an essential factor in regulation of the physiological balance between thrombosis and fibrinolysis. PAI-1 is a labile molecule that exists in four different forms: active, latent, cleaved and target bound form.

    High PAI-1 levels are associated with many cardiovascular diseases. PAI-1 also plays important roles in cell migration, adhesion, senescence, cancer invasion and tissue remodeling. Moreover, the PAI-1 level was extensively validated as the biological prognostic factor in breast cancer and as a marker of a poor prognosis in other cancers. PAI-1 is also one of the plasma biomarkers associated with nonalcoholic fatty liver disease. These associations have made PAI-1 an attractive pharmaceutical target.

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is expressed in endothelial cells (PubMed:2430793, PubMed:3097076) . It is present in plasma, platelets, and hepatocellular carcinoma and fibrosarcoma cells.

    Induction:Up-regulated by coagulation factor Xa (F10) in atrial tissues (PubMed:24041930) . Up-regulated in atrial tissues by rapid pacing resembling atrial fibrillation (PubMed:24041930)

  • Isoforms & Post-Translational Modification

    P05121 has 2 isomers: P05121-1: 45060 Da (predicted); P05121-2: 43404 Da (predicted).
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond

  • Subunit

    Forms a heterodimer with TMPRSS7 (PubMed:15853774). Interacts with VTN (PubMed:7522053). Binds LRP1B; binding is followed by internalization and degradation (PubMed:11384978).

  • SwissProt ID

    P05121

  • Gene ID
    5054 [NCBI]
  • Synonyms

    SERPINE1; PAI1; PLANH1; Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1

  • Research Field

    Cancer

PAI1 Antibody (YA1524) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg), HepG2(lane 3(20μg), HuH-7(lane 4(20μg) and NIH-3T3(lane 5(20μg) using PAI1 Antibody (HY-P81779). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • PAI1 Antibody (YA1524) Product Image
    1/16
  • Experimental Validation Results for PAI1 Antibody (YA1524)
  • PAI1 Antibody (YA1524) Product Image
  • Experimental Validation Results for PAI1 Antibody (YA1524)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg), HepG2(lane 3(20μg), HuH-7(lane 4(20μg) and NIH-3T3(lane 5(20μg) using PAI1 Antibody (HY-P81779). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PAI1 Antibody (YA1524)
  • PAI1 Antibody (YA1524) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20μg), HepG2(lane 3(20μg), HuH-7(lane 4(20μg) and NIH-3T3(lane 5(20μg) using PAI1 Antibody (HY-P81779). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • PAI1 Antibody (YA1524) Product Image WB