PAI1 Antibody (YA1524)

Cat. No.: HY-P81779: Data Sheet COA
SDS
User Guide for Antibodies
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PAI1 Antibody (YA1524) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAI1.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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PAI1 Antibody (YA1524) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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PAI1 Antibody (YA1524) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAI1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P05121

Gene ID

5054 [NCBI]

Immunogen

Recombinant protein of PAI1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF-7(lane 2(20μg), HepG2(lane 3(20μg), HuH-7(lane 4(20μg) and NIH-3T3(lane 5(20μg) using PAI1 Antibody (HY-P81779). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

Function：Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:17912461, PubMed:8481516, PubMed:9207454, PubMed:21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:It is expressed in endothelial cells (PubMed:2430793, PubMed:3097076) . It is present in plasma, platelets, and hepatocellular carcinoma and fibrosarcoma cells.

Induction:Up-regulated by coagulation factor Xa (F10) in atrial tissues (PubMed:24041930) . Up-regulated in atrial tissues by rapid pacing resembling atrial fibrillation (PubMed:24041930)

Isoforms & Post-Translational Modification：P05121 has 2 isomers: P05121-1: 45060 Da (predicted); P05121-2: 43404 Da (predicted).
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond

Subunit：Forms a heterodimer with TMPRSS7 (PubMed:15853774). Interacts with VTN (PubMed:7522053). Binds LRP1B; binding is followed by internalization and degradation (PubMed:11384978).

RRID

AB_3103884

Database

Entrez Gene: 5054 Human ; 24617 Rat

SwissProt: P05121 Human ; P22777 Mouse ; P20961 Rat

OMIM: 613329 Human

Research Field

Cancer

Synonyms

SERPINE1; PAI1; PLANH1; Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1

각종 서류

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

PAI1 Antibody (YA1524) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PAI1 Antibody (YA1524)

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Background

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PAI1 Antibody (YA1524) Related Classifications

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