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Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)

(Synonyms: UBCH 10; UbcH10; Ube2c)
Customer Review

Based on 1 Customer Validation

Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugating Enzyme E2 C.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
  • Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugating Enzyme E2 C.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 20 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 20 kDa
Species Reactivity Database

Entrez Gene: 11065 Human

SwissProt: O00762 Human

Immunogen

A synthesized peptide derived from human UBE2C aa110-179/179.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and COS-7 (lane 4, 15 μg) using Ubiquitin Conjugating Enzyme E2 C Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
    Western blot analysis of extracts from HeLa (lane 2(20μg), HL-60 (lane 3(20μg), THP-1 (lane 4(20μg) and A549 (lane 5(20μg) using Ubiquitin Conjugating Enzyme E2 C Antibody (HY-P82989) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Ubiquitin Conjugating Enzyme E2 C is an Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit

  • Isoforms & Post-Translational Modification

    O00762 has 4 isomers: O00762-1: 19652 Da (predicted); O00762-2: 15765 Da (predicted); O00762-3: 16676 Da (predicted); O00762-4: 17373 Da (predicted).
    Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed

  • Subunit

    Component of the APC/C complex, composed of at least 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa. Within this complex, directly interacts with ANAPC2

  • SwissProt ID

    O00762

  • Gene ID
    11065 [NCBI]
  • Synonyms

    UBCH 10; UbcH10; Ube2c

  • Research Field

    Epigenetics and Nuclear Signaling

Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and COS-7 (lane 4, 15 μg) using Ubiquitin Conjugating Enzyme E2 C Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), HL-60 (lane 3(20μg), THP-1 (lane 4(20μg) and A549 (lane 5(20μg) using Ubiquitin Conjugating Enzyme E2 C Antibody (HY-P82989) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) Product Image
    1/16
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
  • Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) Product Image
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and COS-7 (lane 4, 15 μg) using Ubiquitin Conjugating Enzyme E2 C Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), HL-60 (lane 3(20μg), THP-1 (lane 4(20μg) and A549 (lane 5(20μg) using Ubiquitin Conjugating Enzyme E2 C Antibody (HY-P82989) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
  • Experimental Validation Results for Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734)
  • Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and COS-7 (lane 4, 15 μg) using Ubiquitin Conjugating Enzyme E2 C Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 2(20μg), HL-60 (lane 3(20μg), THP-1 (lane 4(20μg) and A549 (lane 5(20μg) using Ubiquitin Conjugating Enzyme E2 C Antibody (HY-P82989) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) Product Image WB
  • Ubiquitin Conjugating Enzyme E2 C Antibody (YA2734) Product Image WB