GAD67 Antibody (YA1807)

Cat. No.: HY-P82062: Data Sheet COA
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User Guide for Antibodies
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GAD67 Antibody (YA1807) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GAD67.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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GAD67 Antibody (YA1807) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

GAD67 Antibody (YA1807) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GAD67.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 67 kDa;

Observed band size: 67 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q99259

Gene ID

2571 [NCBI]

Immunogen

A synthetic peptide of human GAD67

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using GAD67 Antibody (YA1807). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor; Enzymatically inactive as glutamate decarboxylase

Expression：
Tissue_specificity:It is expressed in brain tissue; it is also expressed in the pancreas, testes, adrenal cortex, and some other endocrine tissues, but not in brain tissue.

Isoforms & Post-Translational Modification：Q99259 has 4 isomers: Q99259-1: 66897 Da (predicted); Q99259-2: Da (predicted); Q99259-3: 25279 Da (predicted); Q99259-4: 47440 Da (predicted).

Subunit：Homodimer

RRID

AB_3104182

Database

Entrez Gene: 2571 Human

SwissProt: Q99259 Human

OMIM: 619124 Human

Research Field

Signal Transduction

Synonyms

Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD 67; GAD1; GAD; GAD67

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

GAD67 Antibody (YA1807) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GAD67 Antibody (YA1807)

GAD67 Antibody (YA1807) Featured Recommendations:

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Background

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GAD67 Antibody (YA1807) Related Classifications

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