Copy Link Print Information

GFAP Antibody (YA416)

1 Cited Publications
カスタマーレビュー

Based on 1 publication(s) in Google Scholar

GFAP Antibody (YA416) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GFAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IP, IF-Tissue, mIHC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • GFAP Antibody (YA416) Product Image
容量
価格（税別）
在庫状況
数量
Amount: USD 0.00
Please select quantity
or
or

* アイテムを追加する前、数量をご選択ください

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

MedChemExpress（MCE）の使用を引用している文献 GFAP Antibody (YA416)

More

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
mIHC Info
mIHC: Multiplex Immunohistochemical
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:100-1:1000 1:200-1:500 1:200-1:500 1:50-1:200 1:1000-1:10000 Use at an assay dependent concentration.

Product Details

製品説明

GFAP Antibody (YA416) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GFAP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Monkey,Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human GFAP.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102091

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • 輸送条件

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GFAP Antibody (YA416)
    Western blot analysis of extracts from U251 (lane2(20μg), Rat brain tissue (lane3(20μg), Mouse brain tissue (lane4(20μg) and HEK293 (lane5(20μg) using GFAP Antibody (HY-P80140). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GFAP Antibody (YA416)
    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GFAP Antibody (YA416)
    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GFAP Antibody (YA416)
    Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody (HY-P80140)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody (HY-P80140) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GFAP Antibody (YA416)
    Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody (HY-P80140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody (HY-P80140) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Expression in cells lacking fibronectin

  • Subunit

    Interacts with SYNM

  • SwissProt ID

    P14136

  • 遺伝子ID
    2670 [NCBI]
  • Research Field

    Epigenetics and Nuclear Signaling

GFAP Antibody (YA416) 関連分類

MOQ
Minimum order quantity
100 mg

お問い合わせ 在庫あり

Other size
お問い合わせ
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U251 (lane2(20μg), Rat brain tissue (lane3(20μg), Mouse brain tissue (lane4(20μg) and HEK293 (lane5(20μg) using GFAP Antibody (HY-P80140). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody (HY-P80140)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody (HY-P80140) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody (HY-P80140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody (HY-P80140) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GFAP Antibody (YA416) Product Image
    1/16
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • GFAP Antibody (YA416) Product Image
  • Experimental Validation Results for GFAP Antibody (YA416)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U251 (lane2(20μg), Rat brain tissue (lane3(20μg), Mouse brain tissue (lane4(20μg) and HEK293 (lane5(20μg) using GFAP Antibody (HY-P80140). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GFAP Antibody (YA416)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GFAP Antibody (YA416)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GFAP Antibody (YA416)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody (HY-P80140)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody (HY-P80140) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GFAP Antibody (YA416)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody (HY-P80140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody (HY-P80140) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • Experimental Validation Results for GFAP Antibody (YA416)
  • GFAP Antibody (YA416) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U251 (lane2(20μg), Rat brain tissue (lane3(20μg), Mouse brain tissue (lane4(20μg) and HEK293 (lane5(20μg) using GFAP Antibody (HY-P80140). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • GFAP Antibody (YA416) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GFAP Antibody (YA416) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue using GFAP Antibody (YA416). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80140, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GFAP Antibody (YA416) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody (HY-P80140)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody (HY-P80140) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GFAP Antibody (YA416) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of U251 cells labeling GFAP with GFAP Antibody (HY-P80140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GFAP Antibody (HY-P80140) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GFAP Antibody (YA416) Product Image WB
  • GFAP Antibody (YA416) Product Image IHC-P
  • GFAP Antibody (YA416) Product Image IHC-P
  • GFAP Antibody (YA416) Product Image ICC
  • GFAP Antibody (YA416) Product Image ICC