Ki67 Antibody (YA322)

Cat. No.: HY-P80506: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Ki67 Antibody (YA322) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ki67.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Ki67 Antibody (YA322) Featured Recommendations:

4 Publications Citing Use of MCE Ki67 Antibody (YA322)

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Ki67 Antibody (YA322) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ki67.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 359 kDa;

Observed band size: 359 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P46013

Gene ID

4288 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Ki67.AA range:1040-1080.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:5000-1:15000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:500
mIHC mIHC: Multiplex Immunohistochemical	1:2000-1:3000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:250
FC FC: Flow Cytometry	1:500-1:1000

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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IHC-P

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Ki67 Antibody (YA322). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80506,1/2000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Ki67 Antibody (YA322). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80506,1/2000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using Ki67 Antibody (YA322). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80506,1/2000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Ki67 Antibody (YA322). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80506,1/2000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using Ki67 Antibody (YA322). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80506,1/2000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Ki67 Antibody (YA322). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80506,1/15000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

RRID

AB_3665685

Database

Entrez Gene: 4288 Human ; 17345 Mouse ; 246042 Rat

SwissProt: P46013 Human ; E9PVX6 Mouse ; Q61769 Mouse ;

OMIM: 176741 Human

Research Field

Cell Biology

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Ki67 Antibody (YA322) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ki67 Antibody (YA322)

Ki67 Antibody (YA322) Featured Recommendations:

4 Publications Citing Use of MCE Ki67 Antibody (YA322)

Product Detail

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Ki67 Antibody (YA322) Related Classifications

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