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CHD8 Antibody (YA6506)

(Synonyms: ATP dependent helicase CHD8 antibody; ATP-dependent helicase CHD8 antibody; Axis duplication inhibitor antibody; Beta catenin binding protein like antibody; CHD 8 antibody; CHD-8 antibody; chd8 antibody; CHD8_HUMAN antibody; Chromodomain helicase DNA binding protein 8 antibody; Chromodomain-helicase-DNA-binding protein 8 antibody; ATP dependent helicase CHD8 antibody; ATP-dependent helicase CHD8 antibody; Axis duplication inhibitor antibody; Beta catenin binding protein like antibody; CHD 8 antibody; CHD-8 antibody; chd8 antibody; CHD8_HUMAN antibody; Chromodomain helicase DNA binding protein 8 antibody; Chromodomain-helicase-DNA-binding protein 8 antibody; DKFZp686N17164 antibody; Duplin antibody; Helicase with SNF2 domain 1 antibody; HELSNF1 antibody; KIAA1564 antibody; )
Customer Review

Based on 1 Customer Validation

CHD8 Antibody (YA6506) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHD8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP, ChIP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CHD8 Antibody (YA6506)
  • CHD8 Antibody (YA6506) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
ChIP Info
ChIP: Chromatin Immunoprecipitation
Dilution Ratio 1:1000 1:50-1:100 1:100-1:200 1-2μg/sample Use 0.5~2 μg for 25 μg of chromatin.

Product Details

Description

CHD8 Antibody (YA6506) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHD8.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 291 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 291 kDa
Immunogen

Recombinant protein within human CHD8 aa 321-570.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CHD8 Antibody (YA6506)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using CHD8 Antibody (HY-P86813) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CHD8 is an ATP-dependent chromatin-remodeling factor, it slides nucleosomes along DNA; nucleosome sliding requires ATP. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. Regulates alternative splicing of a core group of genes involved in neuronal differentiation, cell cycle and DNA repair. Enables H3K36me3-coupled transcription elongation and co-transcriptional RNA processing likely via interaction with HNRNPL[1].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    Q9HCK8 has two isomers: Q9HCK8-1: 290519 Da (predicted); Q9HCK8-2: 262348 Da (predicted).
    Sumoylated

  • Subunit

    Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3

  • SwissProt ID

    Q9HCK8

  • Gene ID
    57680 [NCBI]
  • Synonyms

    ATP dependent helicase CHD8 antibody; ATP-dependent helicase CHD8 antibody; Axis duplication inhibitor antibody; Beta catenin binding protein like antibody; CHD 8 antibody; CHD-8 antibody; chd8 antibody; CHD8_HUMAN antibody; Chromodomain helicase DNA binding protein 8 antibody; Chromodomain-helicase-DNA-binding protein 8 antibody; ATP dependent helicase CHD8 antibody; ATP-dependent helicase CHD8 antibody; Axis duplication inhibitor antibody; Beta catenin binding protein like antibody; CHD 8 antibody; CHD-8 antibody; chd8 antibody; CHD8_HUMAN antibody; Chromodomain helicase DNA binding protein 8 antibody; Chromodomain-helicase-DNA-binding protein 8 antibody; DKFZp686N17164 antibody; Duplin antibody; Helicase with SNF2 domain 1 antibody; HELSNF1 antibody; KIAA1564 antibody;

CHD8 Antibody (YA6506) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using CHD8 Antibody (HY-P86813) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • CHD8 Antibody (YA6506) Product Image
    1/16
  • Experimental Validation Results for CHD8 Antibody (YA6506)
  • CHD8 Antibody (YA6506) Product Image
  • Experimental Validation Results for CHD8 Antibody (YA6506)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using CHD8 Antibody (HY-P86813) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CHD8 Antibody (YA6506)
  • CHD8 Antibody (YA6506) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using CHD8 Antibody (HY-P86813) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • CHD8 Antibody (YA6506) Product Image WB