CaMKII beta Antibody (YA2344)(PBS only)

Cat. No.: HY-P82599A: Data Sheet User Guide for Antibodies
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CaMKII beta Antibody (YA2344) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII beta.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CaMKII beta Antibody (YA2344) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII beta.

Host

Rabbit

Molecular Weight

Predicted band size: 73 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse

SwissProt ID

Q13554

Gene ID

816 [NCBI]

Immunogen

A synthetic peptide of human CaMKII aa209-226.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle (PubMed:16690701). In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise (PubMed:16690701). In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR tH2O2gh phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity). Phosphorylates reticulophagy regulator RETREG1 at 'Ser-151' under endoplasmic reticulum stress conditions which enhances RETREG1 oligomerization and its membrane scission and reticulophagy activity (PubMed:31930741)

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side; Synapse

Expression：
Tissue_specificity:Widely expressed. Expressed tH2O2ghout the human brain. Slightly lower expression in the fetal brain. Also expressed in skeletal muscle.

Induction:Activity is induced in skeletal muscle during exercise

Isoforms & Post-Translational Modification：Q13554 has 8 isomers: Q13554-1: 72678 Da (predicted); Q13554-2: 60387 Da (predicted); Q13554-3: 56379 Da (predicted); Q13554-4: 54074 Da (predicted); Q13554-5: 58009 Da (predicted); Q13554-6: 55223 Da (predicted); Q13554-7: 50955 Da (predicted); Q13554-8: 57938 Da (predicted).
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state

Subunit：CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other (PubMed:14722083, Ref.20).

Research Field

Neuroscience

Synonyms

CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2G; CAMK; CAMK-II; CAMKG; Calcium/calmodulin-dependent protein kinase type II subunit gamma

Documentation

User Guide for Antibodies (1077 KB)