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KLF10 Antibody (YA2349)

(Synonyms: EGR alpha; EGRA; Egral; EGRalpha; Gdnfif; KLF10; mGIF; TIEG; TIEG1)
Customer Review

Based on 1 Customer Validation

KLF10 Antibody (YA2349) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KLF10.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KLF10 Antibody (YA2349)
  • KLF10 Antibody (YA2349) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

KLF10 Antibody (YA2349) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KLF10.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 53 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human KLF10 aa40-100/480.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104705

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KLF10 Antibody (YA2349)
    Western blot analysis of extracts from Jurkat(lane 2(20ug) , MCF-7(lane 3(20ug) and HEK293(lane 4(20ug) using KLF10 Antibody (HY-P82604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    KLF10 transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis. May play a role in the cell cycle regulation

  • Subcellular Localization

    Nucleus

  • Expression


    Induction:By TGFB1 and BMP2

  • Isoforms & Post-Translational Modification

    Q13118 has 4 isomers: Q13118-1: 52555 Da (predicted); Q13118-2: 51424 Da (predicted); Q13118-3: 10600 Da (predicted); Q13118-4: 13702 Da (predicted).
    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation

  • SwissProt ID

    Q13118

  • Gene ID
    7071 [NCBI]
  • Synonyms

    EGR alpha; EGRA; Egral; EGRalpha; Gdnfif; KLF10; mGIF; TIEG; TIEG1

  • Research Field

    Epigenetics and Nuclear Signaling

KLF10 Antibody (YA2349) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , MCF-7(lane 3(20ug) and HEK293(lane 4(20ug) using KLF10 Antibody (HY-P82604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • KLF10 Antibody (YA2349) Product Image
    1/16
  • Experimental Validation Results for KLF10 Antibody (YA2349)
  • KLF10 Antibody (YA2349) Product Image
  • Experimental Validation Results for KLF10 Antibody (YA2349)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , MCF-7(lane 3(20ug) and HEK293(lane 4(20ug) using KLF10 Antibody (HY-P82604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KLF10 Antibody (YA2349)
  • KLF10 Antibody (YA2349) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) , MCF-7(lane 3(20ug) and HEK293(lane 4(20ug) using KLF10 Antibody (HY-P82604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • KLF10 Antibody (YA2349) Product Image WB