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GST Tag Antibody (YA4609)

Customer Review

Based on 1 Customer Validation

GST Tag Antibody (YA4609) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GST Tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, IP, FC, ELISA

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GST Tag Antibody (YA4609)
  • GST Tag Antibody (YA4609) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:10-1:20 1:100-1:1000 1:100-1:1000 1:200-1:400 1:10000

Product Details

Description

GST Tag Antibody (YA4609) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GST Tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Calculated Molecular Weight Predicted band size: 26 kDa
Immunogen

Purified fusion protein with GST protein tag aa 2-210.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GST Tag Antibody (YA4609)
    Western blot analysis of GST tag in recombinant fusion protein (CDC42, Human, E.coli, N-GST, HY-P74258) using GST Tag Antibody (HY-P84912). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    GST Tag is a The GST tag is an affinity tag composed of glutathione S-transferase and is widely used in the purification, soluble expression, and protein-protein interaction studies of recombinant proteins.

GST Tag Antibody (YA4609) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of GST tag in recombinant fusion protein (CDC42, Human, E.coli, N-GST, HY-P74258) using GST Tag Antibody (HY-P84912). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • GST Tag Antibody (YA4609) Product Image
    1/16
  • Experimental Validation Results for GST Tag Antibody (YA4609)
  • GST Tag Antibody (YA4609) Product Image
  • Experimental Validation Results for GST Tag Antibody (YA4609)
    1/1
    MedChemExpress Validation
     Western blot analysis of GST tag in recombinant fusion protein (CDC42, Human, E.coli, N-GST, HY-P74258) using GST Tag Antibody (HY-P84912). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GST Tag Antibody (YA4609)
  • GST Tag Antibody (YA4609) Product Image
    MedChemExpress Validation

    Western blot analysis of GST tag in recombinant fusion protein (CDC42, Human, E.coli, N-GST, HY-P74258) using GST Tag Antibody (HY-P84912). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • GST Tag Antibody (YA4609) Product Image WB