Copy Link Print Information

LTK Antibody (YA3047)

(Synonyms: TYK1)
Customer Review

Based on 1 Customer Validation

LTK Antibody (YA3047) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LTK.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for LTK Antibody (YA3047)
  • Experimental Validation Results for LTK Antibody (YA3047)
  • Experimental Validation Results for LTK Antibody (YA3047)
  • LTK Antibody (YA3047) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

LTK Antibody (YA3047) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LTK.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 92 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 92 kDa
Species Reactivity Database

Entrez Gene: 4058 Human ;

SwissProt: P29376 Human ;

Immunogen

A synthetic peptide of human LTK

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105415

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LTK Antibody (YA3047)
    Western blot analysis of extracts from A549(lane 2(20ug) , C6(lane 3(20ug) and Raji(lane 4(20ug) using LTK Antibody (HY-P83302) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LTK Antibody (YA3047)
    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using LTK Antibody (YA3047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83302, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LTK Antibody (YA3047)
    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using LTK Antibody (YA3047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83302, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    LTK receptor with a tyrosine-protein kinase activity. Following activation by ALKAL1 or ALKAL2 ligands at the cell surface, transduces an extracellular signal into an intracellular response. Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. The exact function of this protein is not known; studies with chimeric proteins demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport[1][2][3][4][5][6].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Expressed in non-hematopoietic cell lines and T- and B-cell lines

  • Isoforms & Post-Translational Modification

    P29376 has 5 isomers: P29376-1: 91681 Da (predicted); P29376-2: 21952 Da (predicted); P29376-3: 48354 Da (predicted); P29376-4: 86213 Da (predicted); P29376-5: 78714 Da (predicted).
    Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity

  • Subunit

    Homodimer; homodimerizes following ligand-binding (PubMed:34646012). Part of a complex including LTK, TNK2 and GRB2, in which GRB2 promotes LTK recruitment by TNK2 (PubMed:19815557)

  • SwissProt ID

    P29376

  • Gene ID
    4058 [NCBI]
  • Synonyms

    TYK1

  • Research Field

    -

LTK Antibody (YA3047) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) , C6(lane 3(20ug) and Raji(lane 4(20ug) using LTK Antibody (HY-P83302) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using LTK Antibody (YA3047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83302, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using LTK Antibody (YA3047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83302, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LTK Antibody (YA3047) Product Image
    1/16
  • Experimental Validation Results for LTK Antibody (YA3047)
  • Experimental Validation Results for LTK Antibody (YA3047)
  • Experimental Validation Results for LTK Antibody (YA3047)
  • LTK Antibody (YA3047) Product Image
  • Experimental Validation Results for LTK Antibody (YA3047)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) , C6(lane 3(20ug) and Raji(lane 4(20ug) using LTK Antibody (HY-P83302) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LTK Antibody (YA3047)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using LTK Antibody (YA3047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83302, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LTK Antibody (YA3047)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using LTK Antibody (YA3047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83302, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LTK Antibody (YA3047)
  • Experimental Validation Results for LTK Antibody (YA3047)
  • Experimental Validation Results for LTK Antibody (YA3047)
  • LTK Antibody (YA3047) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20ug) , C6(lane 3(20ug) and Raji(lane 4(20ug) using LTK Antibody (HY-P83302) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • LTK Antibody (YA3047) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using LTK Antibody (YA3047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83302, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LTK Antibody (YA3047) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using LTK Antibody (YA3047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83302, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LTK Antibody (YA3047) Product Image WB
  • LTK Antibody (YA3047) Product Image IHC-P
  • LTK Antibody (YA3047) Product Image IHC-P