Tyrosine Hydroxylase Antibody (YA3677)
(Synonyms: TYH; DYT14; DYT5b)
Based on 1 Customer Validation
Tyrosine Hydroxylase Antibody (YA3677) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Tyrosine Hydroxylase.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, FC, ELISA
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Reactivity :
Human, Rat
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Tyrosine Hydroxylase Antibody (YA3677) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Tyrosine Hydroxylase.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 59 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 59 kDa
Purified recombinant fragment of human TH (AA: 44-208) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from C6(lane 1(40μg) ) and PC-12(lane 2(40μg) ) using TH antibody(HY-P83980). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10
Background
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Function
Tyrosine hydroxylase (TyrH, TH) is a rate-limiting enzyme in catecholamine biosynthesis and belongs to the family of aromatic amino acid hydroxylases. Tyrosine hydroxylase uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to dopamine. The amino-terminal 150 amino acids of tyrosine hydroxylase form a domain whose structure is involved in regulating the activity of the enzyme. Regulation involves phosphorylation by multiple kinases on 4 different serine residues and dephosphorylation by 2 phosphatases. Tyrosine hydroxylase is inhibited by catecholamine neurotransmitters in a feedback manner. Dopamine competes with tetrahydrobiopterin for binding to tyrosine hydroxylase and interacts with the R domain. Tyrosine hydroxylase activity is regulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins that are thought to be chaperones in mediating the oxidative state of neurons, and proteins that transfer dopamine to secretory vesicles. Tyrosine hydroxylase is modified in the presence of NO. Tyrosine hydroxylase expression maintains dopamine synthesis. Tyrosine hydroxylase is severely lost in Parkinson's disease and slightly lost in Alzheimer's disease.
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Subcellular Localization
Cytoplasm, perinuclear region; Nucleus; Cell projection, axon; Cytoplasm; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle
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Expression
Tissue_specificity:Primarily expressed in the brain and adrenal glands -
Isoforms & Post-Translational Modification
P07101 has 6 isomers: P07101-1: 58600 Da (predicted); P07101-2: 58160 Da (predicted); P07101-3: 55612 Da (predicted); P07101-4: 56052 Da (predicted); P07101-5: 45338 Da (predicted); P07101-6: 44898 Da (predicted).
Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity (PubMed:7901013). Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines (PubMed:15287903). Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway (By similarity). Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network (PubMed:28637871). Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG; this interaction may influence the phosphorylation and dephosphorylation of other sites (PubMed:24947669). Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity (By similarity) -
Subunit
Homotetramer (PubMed:24947669, Ref.18). Interacts (when phosphorylated at Ser-19) with YWHAG; one YWHAG dimer binds to one TH tetramer and this interaction may influence the phosphorylation and dephosphorylation of other sites (PubMed:24947669).
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SwissProt ID
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Synonyms
TYH; DYT14; DYT5b
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Daubner SC, et al. Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys. 2011 Apr 1;508(1):1-12. [Content Brief]
[2]. Zhu Y, et al. Overview of tyrosine hydroxylase in Parkinson's disease. CNS Neurol Disord Drug Targets. 2012 Jun 1;11(4):350-8. [Content Brief]
[3]. Torack RM, et al. Tyrosine hydroxylase-like (TH) immunoreactivity in Parkinson's disease and Alzheimer's disease. J Neural Transm Park Dis Dement Sect. 1992;4(2):165-71. [Content Brief]
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