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CCR5 Antibody (YA4071)

(Synonyms: CCR5; CKR5; CCR-5; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5)
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Based on 1 Customer Validation

CCR5 Antibody (YA4071) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CCR5.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CCR5 Antibody (YA4071)
  • CCR5 Antibody (YA4071) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

CCR5 Antibody (YA4071) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CCR5.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Immunogen

Purified recombinant fragment of human CD195 (AA: extra mix) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718894

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CCR5 Antibody (YA4071)
    Flow cytometric analysis of 1X106 K562 cells labeling CCR5 Antibody (HY-P84374, red). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CCR5 is a human CC chemokine receptor and G protein-coupled receptor that responds to chemokines including RANTES/CCL5, MIP-1α/CCL3, and MIP-1β/CCL4[1]. Mechanistically, ligand-bound CCR5 couples to Gᵢ1 and supports chemokine recognition, receptor activation, immune surveillance, and inflammation[2]. In HIV research models, CCR5 functions as the principal cofactor for entry mediated by envelope glycoproteins of primary macrophage-tropic HIV-1 strains[3]. CCR5, when coexpressed with CD4, enables infection by most primary HIV-1 isolates, whereas CCR3 supports only a more restricted subset, defining an experimentally important distinction from related chemokine receptors[4]. A homozygous CCR5 defect was identified in some multiply exposed individuals resistant to HIV-1 infection, linking receptor expression to viral-entry susceptibility[5]. Compared with related isoforms CCR1 and CCR3, natural LD78β/MIP-1α isoforms show diverging binding capacities that affect CCR5-linked anti-HIV activity and CCR1/CCR3-linked chemotactic responses. For inhibitor studies, maraviroc-bound CCR5 reveals a ligand-binding site distinct from chemokine and gp120 recognition sites, supporting allosteric inhibition of chemokine signaling and viral entry[6]. Maraviroc is a CCR5 antagonist used to block gp120 interaction with CCR5 in HIV/AIDS research and treatment contexts[7].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:It is highly expressed in the spleen, thymus, myeloid cell line THP-1, promyelocytic cell line KG-1a, and CD4+ and CD8+ T cells. Its expression level is moderate in peripheral blood leukocytes and the small intestine. Its expression level is low in the ovary and lung.

    Induction: (Microbial infection) May be down-regulated by human cytomegalovirus/HHV-5 protein UL78

  • Subunit

    Interacts with PRAF2 (PubMed:15757671). Efficient ligand binding to CCL3/MIP-1alpha and CCL4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on Ser-6 is required for efficient binding of CCL4 (PubMed:10383387, PubMed:11733580, PubMed:8663314, PubMed:8699119). Interacts with GRK2 (PubMed:10085131). Interacts with ARRB1 and ARRB2 (PubMed:11448957, PubMed:16144840). Interacts with CNIH4 (PubMed:24405750). Interacts with S100A4; this interaction stimulates T-lymphocyte chemotaxis (PubMed:30713770)

  • SwissProt ID

    P51681

  • Gene ID
    1234 [NCBI]
  • Synonyms

    CCR5; CKR5; CCR-5; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

[1]. Samson M, et al. Molecular cloning and functional expression of a new human CC-chemokine receptor gene. Biochemistry. 1996 Mar 19;35(11):3362-7. [Content Brief]

[2]. Zhang H, et al. Structural basis for chemokine recognition and receptor activation of chemokine receptor CCR5. Nat Commun. 2021 Jul 6;12(1):4151. [Content Brief]

[3]. Deng H, et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature. 1996 Jun 20;381(6584):661-6. [Content Brief]

[4]. Choe H, et al. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996 Jun 28;85(7):1135-48. [Content Brief]

[5]. Liu R, et al. Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection. Cell. 1996 Aug 9;86(3):367-77. [Content Brief]

[6]. Tan Q, et al. Structure of the CCR5 chemokine receptor-HIV entry inhibitor maraviroc complex. Science. 2013 Sep 20;341(6152):1387-90. [Content Brief]

[7]. Xu GG, et al. Chemokine receptor CCR5 antagonist maraviroc: medicinal chemistry and clinical applications. Curr Top Med Chem. 2014;14(13):1504-14. [Content Brief]

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CCR5 Antibody (YA4071) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling CCR5 Antibody (HY-P84374, red). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CCR5 Antibody (YA4071) Product Image
    1/16
  • Experimental Validation Results for CCR5 Antibody (YA4071)
  • CCR5 Antibody (YA4071) Product Image
  • Experimental Validation Results for CCR5 Antibody (YA4071)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling CCR5 Antibody (HY-P84374, red). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CCR5 Antibody (YA4071)
  • CCR5 Antibody (YA4071) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling CCR5 Antibody (HY-P84374, red). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CCR5 Antibody (YA4071) Product Image FC