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VGluT1 Antibody (YA6834)

(Synonyms: BNPI antibody; Brain specific Na (+) dependent inorganic phosphate cotransporter antibody; Brain specific Na dependent inorganic phosphate cotransporter antibody; Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody; Slc17a7 antibody; Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody; Solute carrier family 17 member 7 antibody; Vesicular glutamate transporter 1 antibody; VGLU1_HUMAN antibody; VGLUT 1 antibody; BNPI antibody; Brain specific Na (+) dependent inorganic phosphate cotransporter antibody; Brain specific Na dependent inorganic phosphate cotransporter antibody; Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody; Slc17a7 antibody; Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody; Solute carrier family 17 member 7 antibody; Vesicular glutamate transporter 1 antibody; VGLU1_HUMAN antibody; VGLUT 1 antibody; VGluT1 antibody; )
Customer Review

Based on 1 Customer Validation

VGluT1 Antibody (YA6834) is a Rat -derived and non-conjugated IgG1 monoclonal antibody, targeting to VGluT1.

For research use only. We do not sell to patients.
  • Host:

    Rat

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, IHC-F

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
  • VGluT1 Antibody (YA6834) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:500 1:500

Product Details

Description

VGluT1 Antibody (YA6834) is a Rat -derived and non-conjugated IgG1 monoclonal antibody, targeting to VGluT1.

  • Host Rat
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse, Rat Predicted Reactivity: Cynomolgus monkey,Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3719271

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VGluT1 Antibody (YA6834)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using VGluT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using VGluT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    VGluT1 multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification. Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake. The vesicular K(+)/H(+) antiport activity is electroneutral. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation. The symporter activity is driven by an inside negative membrane potential and is electrogenic. Is necessary for synaptic signaling of visual-evoked responses from photoreceptors[1][2][3][4][5][6][7].

  • Subcellular Localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane,Cell membrane,Synapse, synaptosome

  • Expression


    Tissue_Specificity: Expressed in hippocampus (at protein level). Expressed in the molecular layer of the cerebellum and in retina
    Induction: Oscillates diurnally in synaptic vesicles (at protein level)

  • Isoforms & Post-Translational Modification

    Q3TXX4: 560 amino acids, molecular weight 61637 Da.

  • Subunit

    Interacts with SHANK3

  • SwissProt ID

    Q3TXX4

  • Gene ID
    72961 [NCBI]
  • Synonyms

    BNPI antibody; Brain specific Na (+) dependent inorganic phosphate cotransporter antibody; Brain specific Na dependent inorganic phosphate cotransporter antibody; Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody; Slc17a7 antibody; Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody; Solute carrier family 17 member 7 antibody; Vesicular glutamate transporter 1 antibody; VGLU1_HUMAN antibody; VGLUT 1 antibody; BNPI antibody; Brain specific Na (+) dependent inorganic phosphate cotransporter antibody; Brain specific Na dependent inorganic phosphate cotransporter antibody; Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody; Slc17a7 antibody; Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody; Solute carrier family 17 member 7 antibody; Vesicular glutamate transporter 1 antibody; VGLU1_HUMAN antibody; VGLUT 1 antibody; VGluT1 antibody;

VGluT1 Antibody (YA6834) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using VGluT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using VGluT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • VGluT1 Antibody (YA6834) Product Image
    1/16
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
  • VGluT1 Antibody (YA6834) Product Image
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using VGluT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using VGluT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
  • Experimental Validation Results for VGluT1 Antibody (YA6834)
  • VGluT1 Antibody (YA6834) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using VGluT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • VGluT1 Antibody (YA6834) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using VGluT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • VGluT1 Antibody (YA6834) Product Image IHC-P
  • VGluT1 Antibody (YA6834) Product Image IHC-P