uPA Antibody (YA3248)(PBS only)

Cat. No.: HY-P83503A: Data Sheet User Guide for Antibodies
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uPA Antibody (YA3248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to uPA.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

uPA Antibody (YA3248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to uPA.

Host

Rabbit

Molecular Weight

Predicted band size: 49 kDa;

Observed band size: 49 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P00749

Gene ID

5328 [NCBI]

Immunogen

A synthesized peptide derived from human Urokinase

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Chromatography	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Expressed in the prostate and prostate cancer

Isoforms & Post-Translational Modification：P00749 has 2 isomers: P00749-1: 48523 Da (predicted); P00749-2: 46925 Da (predicted).
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding;Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4

Subunit：Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR. In complex with SERPINE1, interacts with PLAUR/uPAR (PubMed:15053742). Interacts with SORL1 and LRP1, either alone or in complex with SERPINE1; these interactions are abolished in the presence of LRPAP1/RAP (PubMed:15053742). The ternary complex composed of PLAUR-PLAU-PAI1 also interacts with SORLA (PubMed:15053742)

Research Field

Neuroscience

Synonyms

Urokinase-type plasminogen activator; U-plasminogen activator; Upa; PLAU; ATF uPA

Documentation

User Guide for Antibodies (1077 KB)