Syndecan 4 Antibody (YA6792)

Cat. No.: HY-P87099: Data Sheet COA
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Syndecan 4 Antibody (YA6792) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syndecan 4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Syndecan 4 Antibody (YA6792) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syndecan 4.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 22 kDa;

Observed band size: 22 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P31431

Gene ID

6385 [NCBI]

Immunogen

Recombinant protein within human Syndecan 4 Extracellular.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) and MCF7 (lane 3(20μg)) using Syndecan 4 Antibody (HY-P87099) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Cell surface proteoglycan which regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413)

Subcellular Localization：Membrane,Secreted,Secreted

Expression：
Tissue_Specificity: Detected in fibroblasts (at protein level) (PubMed:1500433, PubMed:36213313). Also expressed in epithelial cells (PubMed:1500433)

Isoforms & Post-Translational Modification：P31431 has two isomers: P31431-1: 21642 Da (predicted); P31431-2: 16545 Da (predicted).
Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF丨O-glycosylated; contains both chondroitin sulfate and heparan sulfate

Subunit：Homodimer

Synonyms

Amphiglycan antibody; MGC22217 antibody; OTTHUMP00000031788 antibody; ryudocan amphiglycan antibody; Ryudocan antibody; Ryudocan core protein antibody; SDC 4 antibody; Sdc4 antibody; SDC4_HUMAN antibody; SYND 4 antibody; Amphiglycan antibody; MGC22217 antibody; OTTHUMP00000031788 antibody; ryudocan amphiglycan antibody; Ryudocan antibody; Ryudocan core protein antibody; SDC 4 antibody; Sdc4 antibody; SDC4_HUMAN antibody; SYND 4 antibody; SYND4 antibody; syndecan 4 antibody; syndecan 4 (amphiglycan, ryudocan) antibody; syndecan proteoglycan 4 antibody; Syndecan-4 antibody; Syndecan4 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)