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ALDH1L1 Antibody (YA3566)

(Synonyms: FDH; FTHFD; 10-fTHF; 10-FTHFDH)
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Based on 1 Customer Validation

ALDH1L1 Antibody (YA3566) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ALDH1L1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
  • ALDH1L1 Antibody (YA3566) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

ALDH1L1 Antibody (YA3566) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ALDH1L1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 99 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 99 kDa
Immunogen

Purified recombinant fragment of human ALDH1L1 (AA: 10-222) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
    Western blot analysis of extracts from Mouse liver(lane 1(40μg)and Mouse kidney(lane 2(40μg) ) using ALDH1L1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
    Flow cytometric analysis of 1X10^6 NIH3T3 cells labeling ALDH1L1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    ALDH1L1 is a Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide. May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde[1][2].

  • Subcellular Localization

    Cytoplasm, cytosol

  • Expression


    Tissue_specificity:High expression in liver, pancreas and kidney

  • Isoforms & Post-Translational Modification

    O75891 has 4 isomers: O75891-1: 98829 Da (predicted); O75891-2: 87602 Da (predicted); O75891-3: 99753 Da (predicted); O75891-4: 55394 Da (predicted).
    Phosphopantetheinylation at Ser-354 by AASDHPPT is required for the formyltetrahydrofolate dehydrogenase activity

  • Subunit

    Homotetramer

  • SwissProt ID

    O75891

  • Gene ID
    10840 [NCBI]
  • Synonyms

    FDH; FTHFD; 10-fTHF; 10-FTHFDH

ALDH1L1 Antibody (YA3566) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse liver(lane 1(40μg)and Mouse kidney(lane 2(40μg) ) using ALDH1L1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 NIH3T3 cells labeling ALDH1L1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ALDH1L1 Antibody (YA3566) Product Image
    1/16
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
  • ALDH1L1 Antibody (YA3566) Product Image
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse liver(lane 1(40μg)and Mouse kidney(lane 2(40μg) ) using ALDH1L1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 NIH3T3 cells labeling ALDH1L1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
  • Experimental Validation Results for ALDH1L1 Antibody (YA3566)
  • ALDH1L1 Antibody (YA3566) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse liver(lane 1(40μg)and Mouse kidney(lane 2(40μg) ) using ALDH1L1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ALDH1L1 Antibody (YA3566) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 NIH3T3 cells labeling ALDH1L1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ALDH1L1 Antibody (YA3566) Product Image WB
  • ALDH1L1 Antibody (YA3566) Product Image FC