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p53 DINP1 Antibody (YA249)

(Synonyms: p53DINP1, SIP, Stress induced protein, Teap, TP53DINP1, TP53INP1, TP53INP1A, TP53INP1B)
Customer Review

Based on 1 Customer Validation

p53 DINP1 Antibody (YA249) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p53 DINP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
  • p53 DINP1 Antibody (YA249) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:200

Product Details

Description

p53 DINP1 Antibody (YA249) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p53 DINP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human p53 DINP1.AA range:80-188.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102386

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), A549 (lane 3), RAW264.7 (lane 4), and NIH/3T3 (lane 5) using p53 DINP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
    Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), HepG2(lane 3(20μg), A549(lane 4(20μg) ) using p53 DINP1 (HY-P80259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80259, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    p53 DINP1 is an Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC

  • Subcellular Localization

    Cytoplasm, cytosol; Nucleus; Nucleus, PML body; Cytoplasmic vesicle, autophagosome

  • Expression


    Tissue_specificity:Ubiquitously expressed

  • Subunit

    By adriamycin, gamma irradiation and H(2)O(2), in a p53/TP53-dependent way. At lower levels by UV irradiation. By TP73

  • SwissProt ID

    Q96A56

  • Gene ID
    94241 [NCBI]
  • Synonyms

    p53DINP1, SIP, Stress induced protein, Teap, TP53DINP1, TP53INP1, TP53INP1A, TP53INP1B

  • Research Field

    Cell Biology

p53 DINP1 Antibody (YA249) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), A549 (lane 3), RAW264.7 (lane 4), and NIH/3T3 (lane 5) using p53 DINP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), HepG2(lane 3(20μg), A549(lane 4(20μg) ) using p53 DINP1 (HY-P80259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80259, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • p53 DINP1 Antibody (YA249) Product Image
    1/16
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
  • p53 DINP1 Antibody (YA249) Product Image
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), A549 (lane 3), RAW264.7 (lane 4), and NIH/3T3 (lane 5) using p53 DINP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), HepG2(lane 3(20μg), A549(lane 4(20μg) ) using p53 DINP1 (HY-P80259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80259, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
  • Experimental Validation Results for p53 DINP1 Antibody (YA249)
  • p53 DINP1 Antibody (YA249) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), A549 (lane 3), RAW264.7 (lane 4), and NIH/3T3 (lane 5) using p53 DINP1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • p53 DINP1 Antibody (YA249) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), HepG2(lane 3(20μg), A549(lane 4(20μg) ) using p53 DINP1 (HY-P80259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80259, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • p53 DINP1 Antibody (YA249) Product Image WB
  • p53 DINP1 Antibody (YA249) Product Image WB