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TRIM56 Antibody (YA1384)

(Synonyms: RING finger protein 109; RNF109)
Customer Review

Based on 1 Customer Validation

TRIM56 Antibody (YA1384) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIM56.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • TRIM56 Antibody (YA1384) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

TRIM56 Antibody (YA1384) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIM56.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 81 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 81 kDa
Species Reactivity Database

Entrez Gene: 81844 Human

SwissProt: Q9BRZ2 Human

Immunogen

A synthetic peptide of human TRIM56 aa397-410.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRIM56 Antibody (YA1384)
    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), 293T (lane 4, 15 μg), K562 (lane 5, 15 μg), and MCF-7 (lane 6, 15 μg) using TRIM56 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
    Immunocytochemistry analysis of Hela cells labeling TRIM56 with TRIM56 Antibody (HY-P81639) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TRIM56 Antibody (HY-P81639) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
    Immunocytochemistry analysis of Hela cells labeling TRIM56 with TRIM56 Antibody (HY-P81639) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TRIM56 Antibody (HY-P81639) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    TRIM56 is an E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity by mediating ubiquitination of CGAS and STING1. In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets STING1 to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta. Also mediate monoubiquitination of CGAS, thereby promoting CGAS oligomerization and subsequent activation. Promotes also TNFalpha-induced NF-kappa-B signaling by mediating 'Lys-63'-linked ubiquitination TAK1, leading to enhanced interaction between TAK1 and CHUK/IKKalpha. Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus. Acts as a restriction factor of Zika virus through direct interaction with the viral RNA via its C-terminal region[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Widely expressed (at protein level)

    Induction:Up-regulated by IFN-alpha

  • Isoforms & Post-Translational Modification

    Q9BRZ2 has 3 isomers: Q9BRZ2-1: 81488 Da (predicted); Q9BRZ2-2: 33550 Da (predicted); Q9BRZ2-3: 29185 Da (predicted).
    (Microbial infection) Preferentially ubiquitinated with 'Lys-48' and 'Lys-11'-linked ubiquitin chains by Salmonella effector SopA leading to proteasomal targeting and degradation;Autoubiquitinated

  • Subunit

    Homooligomer. Interacts with TICAM1 (PubMed:22948160)

  • SwissProt ID

    Q9BRZ2

  • Gene ID
    81844 [NCBI]
  • Synonyms

    RING finger protein 109; RNF109

  • Research Field

    Epigenetics and Nuclear Signaling

TRIM56 Antibody (YA1384) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), 293T (lane 4, 15 μg), K562 (lane 5, 15 μg), and MCF-7 (lane 6, 15 μg) using TRIM56 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TRIM56 with TRIM56 Antibody (HY-P81639) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TRIM56 Antibody (HY-P81639) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TRIM56 with TRIM56 Antibody (HY-P81639) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TRIM56 Antibody (HY-P81639) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TRIM56 Antibody (YA1384) Product Image
    1/16
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • TRIM56 Antibody (YA1384) Product Image
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), 293T (lane 4, 15 μg), K562 (lane 5, 15 μg), and MCF-7 (lane 6, 15 μg) using TRIM56 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TRIM56 with TRIM56 Antibody (HY-P81639) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TRIM56 Antibody (HY-P81639) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TRIM56 with TRIM56 Antibody (HY-P81639) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TRIM56 Antibody (HY-P81639) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • Experimental Validation Results for TRIM56 Antibody (YA1384)
  • TRIM56 Antibody (YA1384) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), 293T (lane 4, 15 μg), K562 (lane 5, 15 μg), and MCF-7 (lane 6, 15 μg) using TRIM56 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRIM56 Antibody (YA1384) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TRIM56 with TRIM56 Antibody (HY-P81639) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TRIM56 Antibody (HY-P81639) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TRIM56 Antibody (YA1384) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TRIM56 with TRIM56 Antibody (HY-P81639) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TRIM56 Antibody (HY-P81639) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TRIM56 Antibody (YA1384) Product Image WB
  • TRIM56 Antibody (YA1384) Product Image ICC
  • TRIM56 Antibody (YA1384) Product Image ICC