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LRP1 Antibody (YA306)

(Synonyms: A2MR; alpha 2MR; Alpha 2 macroglobulin receptor; CD91; APR; LRP1; LRP85; TGFBR5)
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Based on 1 publication(s) in Google Scholar

LRP1 Antibody (YA306) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LRP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • LRP1 Antibody (YA306) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:20

Product Details

Description

LRP1 Antibody (YA306) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LRP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 85 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 505 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human LRP1 aa4491-4504.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102690

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LRP1 Antibody (YA306)
    Western blot analysis of extracts from M-heart (lane 1) and M-liver (lane 2) and M-spleen (lane 3) using LRP1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for LRP1 Antibody (YA306)
    Immunocytochemistry analysis of Hela cells labeling LRP1 with LRP1 Antibody (HY-P80746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LRP1 Antibody (HY-P80746) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LRP1 Antibody (YA306)
    Immunocytochemistry analysis of U87 cells labeling LRP1 with LRP1 Antibody (HY-P80746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LRP1 Antibody (HY-P80746) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Low-density lipoprotein receptor-related protein 1 (LRP1) is a multifunctional endocytic and signaling receptor of the LDL receptor family that regulates lipoprotein metabolism, protease clearance, cellular signaling, and tissue homeostasis across multiple organ systems[1][2]. Mechanistically, LRP1 couples rapid ligand internalization with signal transduction, allowing coordinated control of extracellular protein turnover and intracellular responses involved in cell survival, migration, inflammation, and vascular biology[1][2]. Through its broad ligand repertoire, LRP1 participates in key biological processes including endocytosis, phagocytosis, and receptor-mediated clearance pathways that maintain physiological balance in the liver, vasculature, and central nervous system[1][2]. In disease contexts, LRP1 has been extensively studied in neurodegenerative disorders because it contributes to amyloid-β (Aβ) homeostasis through cellular uptake, lysosomal trafficking, and clearance mechanisms[3][4]. Experimental evidence demonstrates that LRP1-mediated endocytosis promotes neuronal Aβ42 uptake and accumulation within lysosomal compartments, highlighting a central role in neuronal Aβ metabolism[3]. LRP1 is also implicated in vascular disease, inflammation, and tissue remodeling through its ability to integrate endocytic and signaling functions within a single receptor system[1][2]. Compared with related LDL receptor family members, LRP1 is distinguished by its exceptionally broad ligand-binding capacity and dual role as both a scavenger receptor and signaling receptor, enabling regulation of diverse physiological pathways[1][2]. Consequently, genetic manipulation of LRP1 has become a widely used experimental approach for investigating receptor-mediated transport, neurodegeneration, vascular biology, and cell-signaling mechanisms[1][3][4].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Membrane, coated pit; Cell membrane; Peripheral membrane protein; Extracellular side; Membrane, coated pit; Cytoplasm; Nucleus; Golgi outpost; Cytoplasm, cytoskeleton, microtubule organizing center

  • Expression


    Tissue_specificity:It is most abundant in the liver, brain, and lungs.

  • Isoforms & Post-Translational Modification

    Q07954 has 2 isomers: Q07954-1: 504606 Da (predicted); Q07954-2: 31631 Da (predicted).
    Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane;The N-terminus is blocked;Phosphorylated on serine and threonine residues;Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1

  • Subunit

    Heterodimer of an 85-kDa membrane-bound carboxyl subunit and a non-covalently attached 515-kDa N-terminal subunit. Intracellular domain interacts with MAFB (By similarity). Found in a complex with PID1/PCLI1, LRP1 and CUBNI (PubMed:17124247). Interacts with SNX17, PID1/PCLI1, PDGF and CUBN. The intracellular domain interacts with SHC1, GULP1 and DAB1. Can weakly interact (via NPXY motif) with DAB2 (via PID domain); the interaction is enhanced by tyrosine phosphorylation of the NPXY motif. Interacts with MDK; promotes neuronal survival (PubMed:10772929). Interacts with LRPAP1; this interaction is followed by rapid internalization (PubMed:15053742, PubMed:16938309, PubMed:32296178). Interacts with uPA/PLAU and PAI1/SERPINE1, either individually or in complex with each other, leading to rapid endocytosis; this interaction is abolished in the presence of LRPAP1/RAP (PubMed:1423604, PubMed:15053742). Also interacts with tPA/PLAT alone or in complex with SERPINE1 (PubMed:15053742). Interacts with the urokinase receptor PLAUR; this interaction leads to PLAUR internalization and is impaired in the presence of SORL1 (PubMed:14764453). Interacts with PDGFB (PubMed:15053742). Interacts with TAU/MAPT, leading to endocytosis; this interaction is reduced in the presence of LRPAP1/RAP (PubMed:32296178). Interacts with IGFBP3; this interaction mediates cell growth inhibition independently of IGF1 (PubMed:9252371). Interacts with ADGRG6 (By similarity)

  • SwissProt ID

    Q07954

  • Gene ID
    4035 [NCBI]
  • Synonyms

    A2MR; alpha 2MR; Alpha 2 macroglobulin receptor; CD91; APR; LRP1; LRP85; TGFBR5

  • Research Field

    Neuroscience

[1]. Strickland DK, et al. Low-density lipoprotein receptor-related protein-1: role in the regulation of vascular integrity. Arterioscler Thromb Vasc Biol. 2014 Mar;34(3):487-98. [Content Brief]

[2]. Fuentealba RA, et al. Low-density lipoprotein receptor-related protein 1 (LRP1) mediates neuronal Abeta42 uptake and lysosomal trafficking. PLoS One. 2010 Jul 29;5(7):e11884. [Content Brief]

LRP1 Antibody (YA306) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from M-heart (lane 1) and M-liver (lane 2) and M-spleen (lane 3) using LRP1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling LRP1 with LRP1 Antibody (HY-P80746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LRP1 Antibody (HY-P80746) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U87 cells labeling LRP1 with LRP1 Antibody (HY-P80746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LRP1 Antibody (HY-P80746) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LRP1 Antibody (YA306) Product Image
    1/16
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • LRP1 Antibody (YA306) Product Image
  • Experimental Validation Results for LRP1 Antibody (YA306)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from M-heart (lane 1) and M-liver (lane 2) and M-spleen (lane 3) using LRP1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for LRP1 Antibody (YA306)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling LRP1 with LRP1 Antibody (HY-P80746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LRP1 Antibody (HY-P80746) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LRP1 Antibody (YA306)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U87 cells labeling LRP1 with LRP1 Antibody (HY-P80746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LRP1 Antibody (HY-P80746) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • Experimental Validation Results for LRP1 Antibody (YA306)
  • LRP1 Antibody (YA306) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from M-heart (lane 1) and M-liver (lane 2) and M-spleen (lane 3) using LRP1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • LRP1 Antibody (YA306) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling LRP1 with LRP1 Antibody (HY-P80746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LRP1 Antibody (HY-P80746) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LRP1 Antibody (YA306) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U87 cells labeling LRP1 with LRP1 Antibody (HY-P80746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LRP1 Antibody (HY-P80746) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LRP1 Antibody (YA306) Product Image WB
  • LRP1 Antibody (YA306) Product Image ICC
  • LRP1 Antibody (YA306) Product Image ICC