2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. LRP1 Antibody (YA306)

LRP1 Antibody (YA306) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LRP1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Documentation

Description

LRP1 Antibody (YA306) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LRP1.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 505 kDa;
Observed band size: 85 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human LRP1 aa4491-4504.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from M-heart (lane 1) and M-liver (lane 2) and M-spleen (lane 3) using LRP1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background
Function：Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells (PubMed:11907044, PubMed:12713657). Required for early embryonic development (By similarity). Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Acts as an LRPAP1 alpha-2-macroglobulin receptor (PubMed:1702392, PubMed:26142438). Acts as TAU/MAPT receptor and controls the endocytosis of TAU/MAPT as well as its subsequent spread (PubMed:32296178). May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission (PubMed:12888553). Also acts as a receptor for IGFBP3 to mediate cell growth inhibition (PubMed:9252371); (Microbial infection) Functions as a receptor for Pseudomonas aeruginosa exotoxin A
Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane, coated pit; Cell membrane; Peripheral membrane protein; Extracellular side; Membrane, coated pit; Cytoplasm; Nucleus; Golgi outpost; Cytoplasm, cytoskeleton, microtubule organizing center
Expression：
Tissue_specificity:It is most abundant in the liver, brain, and lungs.
Isoforms & Post-Translational Modification：Q07954 has 2 isomers: Q07954-1: 504606 Da (predicted); Q07954-2: 31631 Da (predicted).
Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane;The N-terminus is blocked;Phosphorylated on serine and threonine residues;Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1
Subunit：Heterodimer of an 85-kDa membrane-bound carboxyl subunit and a non-covalently attached 515-kDa N-terminal subunit. Intracellular domain interacts with MAFB (By similarity). Found in a complex with PID1/PCLI1, LRP1 and CUBNI (PubMed:17124247). Interacts with SNX17, PID1/PCLI1, PDGF and CUBN. The intracellular domain interacts with SHC1, GULP1 and DAB1. Can weakly interact (via NPXY motif) with DAB2 (via PID domain); the interaction is enhanced by tyrosine phosphorylation of the NPXY motif. Interacts with MDK; promotes neuronal survival (PubMed:10772929). Interacts with LRPAP1; this interaction is followed by rapid internalization (PubMed:15053742, PubMed:16938309, PubMed:32296178). Interacts with uPA/PLAU and PAI1/SERPINE1, either individually or in complex with each other, leading to rapid endocytosis; this interaction is abolished in the presence of LRPAP1/RAP (PubMed:1423604, PubMed:15053742). Also interacts with tPA/PLAT alone or in complex with SERPINE1 (PubMed:15053742). Interacts with the urokinase receptor PLAUR; this interaction leads to PLAUR internalization and is impaired in the presence of SORL1 (PubMed:14764453). Interacts with PDGFB (PubMed:15053742). Interacts with TAU/MAPT, leading to endocytosis; this interaction is reduced in the presence of LRPAP1/RAP (PubMed:32296178). Interacts with IGFBP3; this interaction mediates cell growth inhibition independently of IGF1 (PubMed:9252371). Interacts with ADGRG6 (By similarity)
RRID
Database

Entrez Gene: 4035 Human ; 16971 Mouse ; 299858 Rat

SwissProt: Q07954 Human ; Q91ZX7 Mouse ;

OMIM: 604093 Human

Research Field

Neuroscience
Synonyms
A2MR; alpha 2MR; Alpha 2 macroglobulin receptor; CD91; APR; LRP1; LRP85; TGFBR5
Documentation
SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

LRP1 Antibody (YA306) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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LRP1 Antibody (YA306)
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