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MICU1 Antibody (YA5426)

(Synonyms: Calcium uptake protein 1, mitochondrial; Atopy-related autoantigen CALC; ara CALC; Calcium-binding atopy-related autoantigen 1; allergen Hom s 4)
Customer Review

Based on 1 Customer Validation

MICU1 Antibody (YA5426) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MICU1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • MICU1 Antibody (YA5426) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:100-200 1:200

Product Details

Description

MICU1 Antibody (YA5426) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MICU1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Recombinant Protein of MICU1

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Western blot analysis of extracts from Hela(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) , RAW264.7(lane 5(20ug) using MICU1 Antibody (HY-P85734) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Immunocytochemistry analysis of A431 cells labeling MICU1 with MICU1 Antibody (HY-P85734) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MICU1 Antibody (HY-P85734) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    Immunocytochemistry analysis of Hela cells labeling MICU1 with MICU1 Antibody (HY-P85734) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MICU1 Antibody (HY-P85734) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    MICU1 is a Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains. MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium. At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake. At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel. Also required to protect against manganese toxicity by preventing manganese uptake by MCU: mechanistically, manganese-binding to its EF-hand domains does not induce any conformational change, maintaining MCU pore occlusion. Also acts as a barrier for inhibitors of the MCU channel, such as ruthenium red or its derivative Ru360. Acts as a regulator of mitochondrial cristae structure independently of its ability to regulate the mitochondrial calcium uniporter channel. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG; Isoform that regulates mitochondrial calcium uniporter (MCU) in the skeletal muscle. Compared to other isoforms, this isoform has higher affinity for calcium, promoting mitochondrial calcium uptake at lower calcium concentrations. This allows a rapid response of mitochondrial metabolism and ensures sustained ATP production needed for resistance and strenuous exercise[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29].

  • Subcellular Localization

    Mitochondrion intermembrane space; Mitochondrion inner membrane; Mitochondrion intermembrane space; Mitochondrion inner membrane

  • Expression


    Tissue_specificity:Expressed in epithelial cell lines. Strongly expressed in epidermal keratinocytes and dermal endothelial cells

  • Isoforms & Post-Translational Modification

    Q9BPX6 has 6 isomers: Q9BPX6-1: 54351 Da (predicted); Q9BPX6-2: 45975 Da (predicted); Q9BPX6-3: 54582 Da (predicted); Q9BPX6-4: 32122 Da (predicted); Q9BPX6-5: 31931 Da (predicted); Q9BPX6-6: 54928 Da (predicted).
    Phosphorylation at Ser-122 by AKT1 impairs its maturation and stability;Asymmetric dimethylation at Arg-455 by PRMT1 decreases the calcium sensitivity of MICU1 by promoting interaction with UCP2;Degraded by YME1L1 when not complexed as homodimer or heterodimer (PubMed:36206740). Not degraded when complexed as homodimer or heterodimer; the presence of the interchain disulfide bond protecting MICU1 from degradation by YME1L1 (PubMed:36206740)

  • Subunit

    Heterodimer; disulfide-linked; heterodimerizes with MICU2 or MICU3 (PubMed:24560927, PubMed:28615291, PubMed:31397067, PubMed:32148862, PubMed:32494073, PubMed:32790952). Homodimer; disulfide-linked (PubMed:36206740). Component of the uniplex complex, composed of MCU, EMRE/SMDT1, MICU1 and MICU2 (or MICU3) in a 4:4:1:1 stoichiometry (PubMed:21685886, PubMed:23101630, PubMed:23178883, PubMed:24231807, PubMed:24332854, PubMed:26341627, PubMed:26387864, PubMed:27099988, PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:32862359). The composition of calcium sensors within the uniplex complex can differ depending on tissues: a MICU1 homodimer can be present instead of the MICU1-MICU2 heterodimer in skeletal-muscle and kidney (PubMed:36206740). MICU1 is recruited to the uniplex complex by EMRE/SMDT1, and it associates with MCU at low calcium levels, occluding the pore of the MCU channel (PubMed:30454562, PubMed:30638448, PubMed:32494073, PubMed:37036971, PubMed:37126688). Associates with the MICOS complex (PubMed:37098122). Interacts with SLC25A23 (PubMed:24430870). Interacts with CHCHD4/MIA40; which introduces the interchain disulfide bond with MICU2 (PubMed:26387864). Interacts (when methylated) with UCP2; leading to decrease the calcium sensitivity of MICU1 (PubMed:27642082)

  • SwissProt ID

    Q9BPX6

  • Gene ID
    10367 [NCBI]
  • Synonyms

    Calcium uptake protein 1, mitochondrial; Atopy-related autoantigen CALC; ara CALC; Calcium-binding atopy-related autoantigen 1; allergen Hom s 4

MICU1 Antibody (YA5426) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) , RAW264.7(lane 5(20ug) using MICU1 Antibody (HY-P85734) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling MICU1 with MICU1 Antibody (HY-P85734) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MICU1 Antibody (HY-P85734) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MICU1 with MICU1 Antibody (HY-P85734) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MICU1 Antibody (HY-P85734) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MICU1 Antibody (YA5426) Product Image
    1/16
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • MICU1 Antibody (YA5426) Product Image
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) , RAW264.7(lane 5(20ug) using MICU1 Antibody (HY-P85734) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling MICU1 with MICU1 Antibody (HY-P85734) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MICU1 Antibody (HY-P85734) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MICU1 Antibody (YA5426)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MICU1 with MICU1 Antibody (HY-P85734) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MICU1 Antibody (HY-P85734) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • Experimental Validation Results for MICU1 Antibody (YA5426)
  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) , RAW264.7(lane 5(20ug) using MICU1 Antibody (HY-P85734) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using MICU1 Antibody (HY-P85734, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling MICU1 with MICU1 Antibody (HY-P85734) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MICU1 Antibody (HY-P85734) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MICU1 Antibody (YA5426) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling MICU1 with MICU1 Antibody (HY-P85734) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MICU1 Antibody (HY-P85734) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MICU1 Antibody (YA5426) Product Image WB
  • MICU1 Antibody (YA5426) Product Image IHC-P
  • MICU1 Antibody (YA5426) Product Image IHC-P
  • MICU1 Antibody (YA5426) Product Image IHC-P
  • MICU1 Antibody (YA5426) Product Image IHC-P
  • MICU1 Antibody (YA5426) Product Image IHC-P
  • MICU1 Antibody (YA5426) Product Image IHC-P
  • MICU1 Antibody (YA5426) Product Image ICC
  • MICU1 Antibody (YA5426) Product Image ICC