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MCU Antibody (YA704)

(Synonyms: C10orf42, CCDC109A, MCU, HsMCU, Coiled-coil domain-containing protein 109A)
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Based on 1 publication(s) in Google Scholar

MCU Antibody (YA704) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to MCU.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgM

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    1.Supplied in PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
    2.Supplied in PBS (pH7.4), 0.1% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MCU Antibody (YA704)
  • MCU Antibody (YA704) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF/ICC Info
 
Dilution Ratio 1:5000 1:50 1:50

Product Details

Description

MCU Antibody (YA704) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to MCU.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa,
Species Reactivity Database

Entrez Gene: 90550 Human

SwissProt: Q8NE86 Human

Immunogen

Synthetic peptide corresponding to Human MCU.AA range:51-325 / 351.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgM

RRID

AB_3102354

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
    2.Supplied in PBS (pH7.4), 0.1% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
    Please refer to the lot-specific COA for specific buffer information.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MCU Antibody (YA704)
    Western blot analysis of extracts from HEK293(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane 5(40μg)), using MCU (HY-P80216) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80216, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix (PubMed:21685886, PubMed:21685888, PubMed:22822213, PubMed:22829870, PubMed:22904319, PubMed:23101630, PubMed:23178883, PubMed:23755363, PubMed:24332854, PubMed:24560927, PubMed:26341627, PubMed:29954988, PubMed:29995857, PubMed:30454562, PubMed:30638448, PubMed:31080062, PubMed:32494073, PubMed:32762847, PubMed:33296646, PubMed:37036971, PubMed:37126688). MCU channel activity is regulated by the calcium-sensor subunits of the uniplex MICU1 and MICU2 (or MICU3) (PubMed:24560927, PubMed:26903221, PubMed:30454562, PubMed:30638448, PubMed:32494073, PubMed:32762847, PubMed:37036971, PubMed:37126688). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways (PubMed:21685886, PubMed:21685888, PubMed:22822213, PubMed:22829870, PubMed:22904319, PubMed:23101630, PubMed:23178883, PubMed:23755363, PubMed:24332854, PubMed:24560927, PubMed:26341627, PubMed:29954988, PubMed:32494073, PubMed:32762847). Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (PubMed:22822213). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed:25603276). Participates in mitochondrial permeability transition during ischemia-reperfusion injury (By similarity). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (By similarity). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (By similarity). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed:22829870, PubMed:22904319). Involved in antigen processing and presentation (By similarity)

  • Subcellular Localization

    Mitochondrion inner membrane; Multi-pass membrane protein

  • Expression


    Induction:MCU transcripts are down-regulated by microRNA miR-25 (PubMed:23246404) . Down-regulation by miR-25 may protect cardiomyocytes against oxidative damage in cardiomyocytes (PubMed:25764156)

  • Isoforms & Post-Translational Modification

    Q8NE86 has 3 isomers: Q8NE86-1: 39867 Da (predicted); Q8NE86-2: 36996 Da (predicted); Q8NE86-3: 35159 Da (predicted).
    Phosphorylation by CaMK2 in heart leads to increased MCU current (PubMed:23051746, PubMed:25254481). The regulation of MCU by CaMK2 is however subject to discussion: another group was unable to reproduce these results (PubMed:25254480). Phosphorylated on tyrosines by PTK2B/PYK2, promoting oligomerization (PubMed:24800979);Glutathionylation at Cys-97 in response to reactive oxygen species (ROS) promotes MCU higher-order assembly, leading to constitutive activation of the MCU channel and mitochondrial calcium overload;Undergoes proteolytic degradation by SPG7

  • Subunit

    Homotetramer (PubMed:32494073). Component of the uniplex complex, composed of MCU, EMRE/SMDT1, MICU1 and MICU2 (or MICU3) in a 4:4:1:1 stoichiometry (PubMed:24231807, PubMed:26341627, PubMed:31080062, PubMed:32494073, PubMed:32862359). Interacts with CCDC109B/MCUB; this inhibits channel activity (PubMed:27184846). Interacts with MCUR1 (PubMed:23178883, PubMed:26341627, PubMed:26976564, PubMed:27184846). Interactions with MICU1 and MCUR1 are mutually exclusive (PubMed:23178883). Interacts with SLC25A23 (PubMed:24430870)

  • SwissProt ID

    Q8NE86

  • Gene ID
    90550 [NCBI]
  • Synonyms

    C10orf42, CCDC109A, MCU, HsMCU, Coiled-coil domain-containing protein 109A

  • Research Field

    Cardiovascular

MCU Antibody (YA704) Related Classifications

100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MCU Antibody (YA704) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane 5(40μg)), using MCU (HY-P80216) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80216, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MCU Antibody (YA704) Product Image
    1/16
  • Experimental Validation Results for MCU Antibody (YA704)
  • MCU Antibody (YA704) Product Image
  • MCU Antibody (YA704) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HEK293(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane 5(40μg)), using MCU (HY-P80216) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80216, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MCU Antibody (YA704) Product Image WB