NCE2 Antibody (YA3291)(PBS only)

Cat. No.: HY-P83546A: Data Sheet User Guide for Antibodies
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NCE2 Antibody (YA3291) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCE2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NCE2 Antibody (YA3291) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCE2.

Host

Rabbit

Molecular Weight

Predicted band size: 21 kDa;

Observed band size: 21 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q969M7

Gene ID

140739 [NCBI]

Immunogen

Recombinant protein of human NCE2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:200
FC FC: Flow Cytometry	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins (PubMed:19250909, PubMed:23201271). Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5) (PubMed:19250909, PubMed:23201271, PubMed:23300442). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B (PubMed:19250909, PubMed:23201271). Mediates neddylation of the CUL9-RBX1 complex (PubMed:38605244); (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5)

Expression：
Tissue_specificity:Widely expressed (at protein level)

Isoforms & Post-Translational Modification：Q969M7 has 7 isomers: Q969M7-1: 21077 Da (predicted); Q969M7-2: 23760 Da (predicted); Q969M7-3: 17500 Da (predicted); Q969M7-4: 13513 Da (predicted); Q969M7-5: 11450 Da (predicted); Q969M7-6: 18619 Da (predicted); Q969M7-7: 18158 Da (predicted).
The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins

Subunit：Interacts with UBA3 and RBX2 (PubMed:19250909). Interacts (N-terminally acetylated form) with (via DCUN1 domain) DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5 (PubMed:23201271)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2

Documentation

User Guide for Antibodies (1077 KB)