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MEF2A Antibody (YA3209)

(Synonyms: mef2; ADCAD1; RSRFC4; RSRFC9)
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Based on 1 Customer Validation

MEF2A Antibody (YA3209) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEF2A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MEF2A Antibody (YA3209)
  • MEF2A Antibody (YA3209) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

MEF2A Antibody (YA3209) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEF2A.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human MEF2A

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MEF2A Antibody (YA3209)
    Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), Hela (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using MEF2A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    MEF2A transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:MEF2 and MEFA isoforms are expressed only in skeletal muscle, cardiac muscle, and brain tissue. RSRFC4 and RSRFC9 isoforms are expressed in all tissues tested.

  • Isoforms & Post-Translational Modification

    Q02078 has 8 isomers: Q02078-1: 54811 Da (predicted); Q02078-2: 54442 Da (predicted); Q02078-3: 53596 Da (predicted); Q02078-4: 53227 Da (predicted); Q02078-5: 53852 Da (predicted); Q02078-6: 53483 Da (predicted); Q02078-7: 46529 Da (predicted); Q02078-8: 45570 Da (predicted).
    Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF;Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated with SUMO1 and, to a lesser extent with SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis (By similarity);Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure;Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation

  • Subunit

    Binds DNA as a homo- or heterodimer. Dimerizes with MEF2D.

  • SwissProt ID

    Q02078

  • Gene ID
    4205 [NCBI]
  • Synonyms

    mef2; ADCAD1; RSRFC4; RSRFC9

  • Research Field

    Signal Transduction

MEF2A Antibody (YA3209) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), Hela (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using MEF2A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MEF2A Antibody (YA3209) Product Image
    1/16
  • Experimental Validation Results for MEF2A Antibody (YA3209)
  • MEF2A Antibody (YA3209) Product Image
  • Experimental Validation Results for MEF2A Antibody (YA3209)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), Hela (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using MEF2A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MEF2A Antibody (YA3209)
  • MEF2A Antibody (YA3209) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), Hela (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using MEF2A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MEF2A Antibody (YA3209) Product Image WB