IL-1 beta Antibody (YA5879)
(Synonyms: IL-1; IL1F2; IL1beta; IL1-BETA)
Based on 1 Customer Validation
IL-1 beta Antibody (YA5879) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-1 beta.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IP, FC
-
Reactivity :
Mouse
-
Formulation:
Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
FC
FC: Flow Cytometry
|
IP
IP: Immunoprecipitation
|
|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:50 | 1:50 |
Product Details
IL-1 beta Antibody (YA5879) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-1 beta.
-
Host Rabbit
-
Clonality Monoclonal
-
Species ReactivityMouse
-
Observed Molecular WeightObserved band size: 17 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 31 kDa
Entrez Gene: 3553 Human ; 16176 Mouse
SwissProt: P01584 Human ; P10749 Mouse ; Q63264 Rat
OMIM: 147720 Human
A synthesized peptide derived from human IL1 beta.
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Interleukin-1β (IL-1β) is one of the pro-inflammatory cytokines and is produced and secreted by a variety of cell types although the vast majority of studies have focussed on its production within cells of the innate immune system, such as monocytes and macrophages. IL-1β is produced as inactive pro-IL-1β (encoded by pro-Il-1b) in response to inflammatory stimuli, including both microbial products and endogenous danger-associated molecules. IL-1β could regulate the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. IL-1β also plays a significant regulator of reproduction in females[1][2].
-
Subcellular Localization
Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome
-
Expression
Tissue_specificity:Expression (protein level) in activated monocytes/macrophages.
Induction:By LPS (PubMed:15192144) . Transcription and translation induced by M.tuberculosis and a number of different M.tuberculosis components in macrophages; EsxA is the most potent activator tested (at protein level) (PubMed:20148899) . In pancreatic islets, release is increased by high glucose treatment. In pancreatic islets and macrophages, release is also increased by endocannabinoid anandamide/AEA (PubMed:23955712) -
Isoforms & Post-Translational Modification
1) IL1beta (P01584): Number of amino acids: 269; Molecular weight (Da): 30,748
2) Mouse IL1beta (P10749): Number of amino acids: 269; Molecular weight (Da): 30,931
3) Rat IL1beta (a63264): Number of amino acids: 268; Molecular weight (Da): 30,644 -
Subunit
Monomer. In its precursor form, weakly interacts with full-length MEFV; the mature cytokine does not interact at all (PubMed:17431422). Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling (PubMed:29030430). Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form (PubMed:32272059). Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC (PubMed:32272059). Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC (PubMed:32272059)
-
SwissProt ID
-
Synonyms
IL-1; IL1F2; IL1beta; IL1-BETA
-
Research Field
Immunology
Documentation
References
[1]. Jan Petrasek, et al. IL-1 receptor antagonist ameliorates inflammasome-dependent alcoholic steatohepatitis in mice. J Clin Invest. 2012 Oct;122(10):3476-89. [Content Brief]
[2]. Karina Zitta, et al. Interleukin-1beta regulates cell proliferation and activity of extracellular matrix remodelling enzymes in cultured primary pig heart cells. Biochem Biophys Res Commun. 2010 Sep 3;399(4):542-7. [Content Brief]
Powered by Bioz
