CD31 Antibody (YA6314)
CD31 Antibody (YA6314) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD31.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:1000-5000 | 1:200-1000 | 1:5000-20000 | 1:50-200 |
Product Details
CD31 Antibody (YA6314) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD31.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 83 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD31 cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis; Does not protect against apoptosis[1][2][3][4][5].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Membrane raft; Cell junction; Cell junction
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Expression
Tissue_specificity:This protein is expressed on platelets and leukocytes, primarily concentrated at the boundaries between endothelial cells (PubMed:18388311, PubMed:21464369) . It is also expressed (protein level) in human umbilical vein endothelial cells (HUVECs) (PubMed:17580308, PubMed:19342684) . It is also expressed (protein level) on neutrophils (PubMed:17580308) . The long subtype predominated in all tissues examined (PubMed:12433657) . The Δ12 subtype was detected only in the trachea (PubMed:12433657) . The Δ14-15 subtype was detected only in the lungs (PubMed:12433657) . The Delta14 subtype was detected in all tissues examined, with the strongest expression in the heart (PubMed:12433657) . Delta15 is expressed (protein level) in the brain, testes, ovaries, platelet cells, human umbilical vein endothelial cells (HUVECs) , Jurkat T-cell leukemia, human erythroleukemia (HEL) , and U-937 histiocytic lymphoma cell lines (PubMed:12433657, PubMed:18388311) . -
Isoforms & Post-Translational Modification
P16284 has 6 isomers: P16284-1: 82522 Da (predicted); P16284-2: 80465 Da (predicted); P16284-3: 80193 Da (predicted); P16284-4: 80405 Da (predicted); P16284-5: 79247 Da (predicted); P16284-6: 81364 Da (predicted).
Phosphorylated on Ser and Tyr residues after cellular activation by src kinases (PubMed:18710921, PubMed:19342684, PubMed:21464369, PubMed:9298995). Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690 (PubMed:21464369). Constitutively phosphorylated on Ser-734 in resting platelets (PubMed:21464369). Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation (PubMed:18710921);Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts -
Subunit
Trans-homodimer (via Ig-like C2-type 1 and Ig-like C2-type 2 domains); trans-homodimerization is required for cell-cell interaction (PubMed:26702061, PubMed:27958302). Forms a complex with BDKRB2 and GNAQ (PubMed:18672896). Interacts with BDKRB2 and GNAQ (PubMed:18672896). Interacts with PTPN11; Tyr-713 is critical for PTPN11 recruitment (PubMed:18388311, PubMed:19342684). Interacts with FER (By similarity). Interacts (via Ig-like C2-type domain 6) with CD177; the interaction is Ca(2+)-dependent; the interaction is direct (PubMed:17580308)
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SwissProt ID
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