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RBP1 Antibody (YA7823)

(Synonyms: CRABP-I; CRBP; CRBP1; CRBPI; RBPC)
Customer Review

Based on 1 Customer Validation

RBP1 Antibody (YA7823) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to RBP1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • RBP1 Antibody (YA7823) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000-3000 1:150-500 1:100-250 1:100

Product Details

Description

RBP1 Antibody (YA7823) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to RBP1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 15.7 kDa
Immunogen

Full length human recombinant protein of human RBP1 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RBP1 Antibody (YA7823)
    Western blot analysis of extracts from NIH-3T3(lane 2, 20μg), C2C12(lane 3, 20μg), MCF-7(lane 4, 20μg) and HT-29(lane 5, 20μg) using RBP1 Antibody (HY-P88139). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RBP1 Antibody (YA7823)
    Immunocytochemistry analysis of A431 cells labeling RBP1 with RBP1 Antibody (HY-P88139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RBP1 Antibody (HY-P88139) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RBP1 Antibody (YA7823)
    Immunocytochemistry analysis of HepG2 cells labeling RBP1 with RBP1 Antibody (HY-P88139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RBP1 Antibody (HY-P88139) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    RBP1 is a Cytoplasmic retinol-binding protein. Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis[1][2][3][4].

  • Subcellular Localization

    Cytoplasm,Lipid droplet

  • Expression


    Tissue_Specificity: Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver

  • Isoforms & Post-Translational Modification

    P09455 has three isomers: P09455-1: 15850 Da (predicted); P09455-2: 17104 Da (predicted); P09455-3: 17523 Da (predicted).

  • Subunit

    Interacts (only as retinol-free apoprotein) with STRA6

  • SwissProt ID

    P09455

  • Synonyms

    CRABP-I; CRBP; CRBP1; CRBPI; RBPC

RBP1 Antibody (YA7823) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH-3T3(lane 2, 20μg), C2C12(lane 3, 20μg), MCF-7(lane 4, 20μg) and HT-29(lane 5, 20μg) using RBP1 Antibody (HY-P88139). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling RBP1 with RBP1 Antibody (HY-P88139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RBP1 Antibody (HY-P88139) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling RBP1 with RBP1 Antibody (HY-P88139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RBP1 Antibody (HY-P88139) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RBP1 Antibody (YA7823) Product Image
    1/16
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • RBP1 Antibody (YA7823) Product Image
  • Experimental Validation Results for RBP1 Antibody (YA7823)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH-3T3(lane 2, 20μg), C2C12(lane 3, 20μg), MCF-7(lane 4, 20μg) and HT-29(lane 5, 20μg) using RBP1 Antibody (HY-P88139). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RBP1 Antibody (YA7823)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling RBP1 with RBP1 Antibody (HY-P88139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RBP1 Antibody (HY-P88139) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RBP1 Antibody (YA7823)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling RBP1 with RBP1 Antibody (HY-P88139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RBP1 Antibody (HY-P88139) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • Experimental Validation Results for RBP1 Antibody (YA7823)
  • RBP1 Antibody (YA7823) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH-3T3(lane 2, 20μg), C2C12(lane 3, 20μg), MCF-7(lane 4, 20μg) and HT-29(lane 5, 20μg) using RBP1 Antibody (HY-P88139). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • RBP1 Antibody (YA7823) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling RBP1 with RBP1 Antibody (HY-P88139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RBP1 Antibody (HY-P88139) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RBP1 Antibody (YA7823) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling RBP1 with RBP1 Antibody (HY-P88139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RBP1 Antibody (HY-P88139) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RBP1 Antibody (YA7823) Product Image WB
  • RBP1 Antibody (YA7823) Product Image ICC
  • RBP1 Antibody (YA7823) Product Image ICC