HUWE1 Antibody (YA6600)
(Synonyms: ARF binding protein 1 antibody; ARF BP1 antibody; ARF-binding protein 1 antibody; ARF-BP1 antibody; ARFBP1 antibody; BJ-HCC-24 tumor antigen antibody; E3 ubiquitin protein ligase HUWE1 antibody; E3 ubiquitin-protein ligase HUWE1 antibody; HECT antibody; HECT domain protein LASU1 antibody; ARF binding protein 1 antibody; ARF BP1 antibody; ARF-binding protein 1 antibody; ARF-BP1 antibody; ARFBP1 antibody; BJ-HCC-24 tumor antigen antibody; E3 ubiquitin protein ligase HUWE1 antibody; E3 ubiquitin-protein ligase HUWE1 antibody; HECT antibody; HECT domain protein LASU1 antibody; HECT UBA and WWE domain containing protein 1 antibody; HectH9 antibody; Homologous to E6AP carboxyl terminus homologous protein 9 antibody; Huwe1 antibody; HUWE1_HUMAN antibody; Ib772 antibody; Large structure of UREB1 antibody; LASU1 antibody; Mcl 1 ubiquitin ligase E3 antibody; Mcl-1 ubiquitin ligase E3 antibody; Mule antibody; UBA and WWE domain-containing protein 1 antibody; Upstream regulatory element-binding protein 1 antibody; URE-B1 antibody; URE-binding protein 1 antibody; UREB1 antibody; )HUWE1 Antibody (YA6600) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HUWE1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:1000 | 1:100 | 1:1000 |
Product Details
HUWE1 Antibody (YA6600) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HUWE1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 482.7 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 482.7 kDa
Synthetic peptide within rat HUWE1 aa 2,701-2,750 / 4,378.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
HUWE1 (HECT, UBA, and WWE domain-containing E3 ubiquitin ligase 1) functions as a versatile E3 ligase that catalyzes mono- and polyubiquitination of multiple substrates, thereby regulating protein stability and cellular homeostasis[1][2]. Mechanistically, HUWE1 controls key signaling pathways including N-Myc-DLL1-NOTCH1, AMBRA1-mediated mitophagy, and DNA damage responses via Chk1 regulation, impacting cell proliferation, apoptosis, and stress signaling[3][4][5]. In disease models, HUWE1 exhibits context-dependent roles: it suppresses glioblastoma progression by degrading N-Myc, modulates MYC-dependent proliferation in multiple myeloma, and influences neuronal development and intellectual disability through regulation of neural progenitor proliferation, differentiation, and GABAergic transmission[3][6][7][8]. Compared with related E3 ligases, HUWE1 possesses a giant HECT domain with substrate-binding rings, enabling recognition of diverse substrates including Mcl-1, DDIT4, and APOBEC3 proteins, which distinguishes its substrate specificity and pleiotropic effects[9][10][11]. In experimental applications, HUWE1 can be manipulated using rAAV-based gene delivery, small molecule inhibitors, and inducible knockdown systems, providing tools to modulate tumor growth, apoptosis, and mitochondrial quality control[3][10][7][5]. Collectively, HUWE1 acts as a central node integrating ubiquitination-dependent regulation across oncogenic, apoptotic, neuronal, and antiviral pathways, offering multiple avenues for mechanistic and therapeutic studies.
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Subcellular Localization
Cytoplasm,Nucleus,Mitochondrion
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Expression
Tissue_Specificity: Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas -
Isoforms & Post-Translational Modification
Q7Z6Z7 has three isomers: Q7Z6Z7-1: 481891 Da (predicted); Q7Z6Z7-2: 480198 Da (predicted); Q7Z6Z7-3: 481018 Da (predicted).
Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation -
Subunit
Interacts with isoform p14ARF of CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity
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SwissProt ID
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Synonyms
ARF binding protein 1 antibody; ARF BP1 antibody; ARF-binding protein 1 antibody; ARF-BP1 antibody; ARFBP1 antibody; BJ-HCC-24 tumor antigen antibody; E3 ubiquitin protein ligase HUWE1 antibody; E3 ubiquitin-protein ligase HUWE1 antibody; HECT antibody; HECT domain protein LASU1 antibody; ARF binding protein 1 antibody; ARF BP1 antibody; ARF-binding protein 1 antibody; ARF-BP1 antibody; ARFBP1 antibody; BJ-HCC-24 tumor antigen antibody; E3 ubiquitin protein ligase HUWE1 antibody; E3 ubiquitin-protein ligase HUWE1 antibody; HECT antibody; HECT domain protein LASU1 antibody; HECT UBA and WWE domain containing protein 1 antibody; HectH9 antibody; Homologous to E6AP carboxyl terminus homologous protein 9 antibody; Huwe1 antibody; HUWE1_HUMAN antibody; Ib772 antibody; Large structure of UREB1 antibody; LASU1 antibody; Mcl 1 ubiquitin ligase E3 antibody; Mcl-1 ubiquitin ligase E3 antibody; Mule antibody; UBA and WWE domain-containing protein 1 antibody; Upstream regulatory element-binding protein 1 antibody; URE-B1 antibody; URE-binding protein 1 antibody; UREB1 antibody;
Documentation
References
[1]. Yuan Y, et al. The E3 ubiquitin ligase HUWE1 acts through the N-Myc-DLL1-NOTCH1 signaling axis to suppress glioblastoma progression. Cancer Commun (Lond). 2022 Sep;42(9):868-886. [Content Brief]
[2]. Kurokawa M, et al. A network of substrates of the E3 ubiquitin ligases MDM2 and HUWE1 control apoptosis independently of p53. Sci Signal. 2013 May 7;6(274):ra32. [Content Brief]
[3]. Kao SH, et al. Ubiquitination by HUWE1 in tumorigenesis and beyond. J Biomed Sci. 2018 Sep 4;25(1):67. [Content Brief]
[4]. Giles AC, et al. Roles of the HUWE1 ubiquitin ligase in nervous system development, function and disease. Neural Dev. 2020 Apr 26;15(1):6. [Content Brief]
[5]. Thompson JW, et al. Quantitative Lys-ϵ-Gly-Gly (diGly) proteomics coupled with inducible RNAi reveals ubiquitin-mediated proteolysis of DNA damage-inducible transcript 4 (DDIT4) by the E3 ligase HUWE1. J Biol Chem. 2014 Oct 17;289(42):28942-55. [Content Brief]
[6]. Cassidy KB, et al. Direct regulation of Chk1 protein stability by E3 ubiquitin ligase HUWE1. FEBS J. 2020 May;287(10):1985-1999. [Content Brief]
[7]. Qi L, et al. The giant E3 ligase HUWE1 is linked to tumorigenesis, spermatogenesis, intellectual disability, and inflammatory diseases. Front Cell Infect Microbiol. 2022 Jul 22;12:905906. [Content Brief]
[8]. Crawford LJ, et al. The E3 ligase HUWE1 inhibition as a therapeutic strategy to target MYC in multiple myeloma. Oncogene. 2020 Jul;39(27):5001-5014. [Content Brief]
[9]. Schwartz I, et al. Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity. Nat Commun. 2026 Jan 19;17(1):1723. [Content Brief]
[10]. Di Rita A, et al. HUWE1 E3 ligase promotes PINK1/PARKIN-independent mitophagy by regulating AMBRA1 activation via IKKα. Nat Commun. 2018 Sep 14;9(1):3755. [Content Brief]
[11]. Opperman KJ, et al. The HECT Family Ubiquitin Ligase EEL-1 Regulates Neuronal Function and Development. Cell Rep. 2017 Apr 25;19(4):822-835. [Content Brief]
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