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MBNL1 Antibody (YA2601)

(Synonyms: EXP; EXP35; EXP40; EXP42; MBNL; MBNL1)
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Based on 1 Customer Validation

MBNL1 Antibody (YA2601) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBNL1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MBNL1 Antibody (YA2601)
  • MBNL1 Antibody (YA2601) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

MBNL1 Antibody (YA2601) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBNL1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human MBNL1 aa330-388/388.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MBNL1 Antibody (YA2601)
    Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2), 293T (lane 3), HepG2 (lane 4), mouse heart (lane 5, Negative), and Ramos (lane 6) using MBNL1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    MBNL1 mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. Together with RNA binding proteins RBPMS and RBFOX2, activates vascular smooth muscle cells alternative splicing events. Regulates NCOR2 alternative splicing[1].

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasmic granule

  • Expression


    Tissue_specificity:Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level) . Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas

  • Isoforms & Post-Translational Modification

    Q9NR56 has 7 isomers: Q9NR56-1: 41817 Da (predicted); Q9NR56-2: 39831 Da (predicted); Q9NR56-3: 33050 Da (predicted); Q9NR56-4: 34211 Da (predicted); Q9NR56-5: 40992 Da (predicted); Q9NR56-6: 37074 Da (predicted); Q9NR56-7: 36992 Da (predicted).

  • Subunit

    Interacts with DDX1 and YBX1. Interacts with HNRNPH1; the interaction in RNA-independent. Interacts with RBPMS; the interaction allows cooperative assembly of RNA-bound stable cell-specific alternative splicing regulatory complexes (PubMed:37548402)

  • SwissProt ID

    Q9NR56

  • Gene ID
    4154 [NCBI]
  • Synonyms

    EXP; EXP35; EXP40; EXP42; MBNL; MBNL1

  • Research Field

    -

MBNL1 Antibody (YA2601) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2), 293T (lane 3), HepG2 (lane 4), mouse heart (lane 5, Negative), and Ramos (lane 6) using MBNL1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MBNL1 Antibody (YA2601) Product Image
    1/16
  • Experimental Validation Results for MBNL1 Antibody (YA2601)
  • MBNL1 Antibody (YA2601) Product Image
  • Experimental Validation Results for MBNL1 Antibody (YA2601)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2), 293T (lane 3), HepG2 (lane 4), mouse heart (lane 5, Negative), and Ramos (lane 6) using MBNL1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for MBNL1 Antibody (YA2601)
  • MBNL1 Antibody (YA2601) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2), 293T (lane 3), HepG2 (lane 4), mouse heart (lane 5, Negative), and Ramos (lane 6) using MBNL1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MBNL1 Antibody (YA2601) Product Image WB