Fyn Antibody (YA5237)
(Synonyms: C syn protooncogene; Fyn; FYN oncogene related to SRC FGR YES; FYN_HUMAN; MGC45350; OKT3 induced calcium influx regulator; P59 FYN; p59-Fyn; Protein tyrosine kinase fyn; Proto oncogene tyrosine protein kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; Protooncogene Syn; SLK; Src like kinase; Src yes related novel gene; Src-like kinase; Src/yes related novel; Src/yes related novel gene; SYN; Tyrosine kinase p59fyn T; Tyrosine kinase p59fyn; T; Tyrosine-protein kinase Fyn.)Fyn Antibody (YA5237) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Fyn.
-
Host:
Mouse
-
Application:
WB, ICC/IF
-
Reactivity :
Human, Monkey, Rat, Mouse
-
Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:500 | 1:50 |
Product Details
Fyn Antibody (YA5237) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Fyn.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman, Monkey, Rat, Mouse
-
Observed Molecular WeightObserved band size: 59 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Purified recombinant human Fyn protein fragments expressed in E.coli.
affinity chromatography.
Non-conjugated
Unmodified
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Fyn is a Src family tyrosine kinase represented in brain by the neuronal isoform p59FynB, where Src and Fyn constitute the two main tyrosine kinases involved in neuronal growth and myelination[1]. Mechanistically, Fyn participates in Src-family signaling modules that regulate inflammation, apoptosis, neuroinflammation, and epileptogenesis in experimental systems[2][3]. In pulmonary endothelial inflammation models, fyn knockdown increased caspase-3 activity, reduced viable cell numbers, and intensified LPS-induced lung injury in fyn−/− mice, identifying Fyn as a negative regulator of inflammation-induced apoptosis[2]. In temporal lobe epilepsy models, the Fyn-PKCδ pathway linked status epilepticus to neuroinflammation and epileptogenesis, while saracatinib reduced seizures, gliosis, neurodegeneration, phosphorylated Fyn/Src-419, PKCδ-tyr311, nitrooxidative stress, and proinflammatory cytokines[3]. Compared with related isoforms, Fyn showed an opposite endothelial apoptosis phenotype to Yes, because yes knockdown lowered caspase-3 activity and increased viable cells under cytomix stimulation[2]. For experimental applications, saracatinib functions as a Src/Fyn tyrosine kinase inhibitor for probing epileptogenesis-associated neuroinflammatory and nitrooxidative pathways[3].
-
Subcellular Localization
Cytoplasm; Nucleus; Cell membrane; Perikaryon
-
Expression
Tissue_specificity:Isomer 1 is highly expressed in the brain. Isomer 2 is expressed in hematopoietic lineage cells, especially in T lymphocytes. -
Isoforms & Post-Translational Modification
P06241 has 3 isomers: P06241-1: 60762 Da (predicted); P06241-2: 60141 Da (predicted); P06241-3: 54513 Da (predicted).
Autophosphorylated at Tyr-420 (By similarity). Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (PubMed:1699196). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation (PubMed:1533589). Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus (PubMed:15537652). Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling (PubMed:22080863). Phosphorylated at tyrosine residues, which can be enhanced by NTN1 (By similarity);Palmitoylated. Palmitoylation at Cys-3 and Cys-6, probably by ZDHHC21, regulates subcellular location -
Subunit
Interacts (via its SH3 domain) with PIK3R1 and PRMT8. Interacts with FYB1, PAG1, and SH2D1A. Interacts with CD79A (tyrosine-phosphorylated form); the interaction increases FYN activity.
-
SwissProt ID
-
Synonyms
C syn protooncogene; Fyn; FYN oncogene related to SRC FGR YES; FYN_HUMAN; MGC45350; OKT3 induced calcium influx regulator; P59 FYN; p59-Fyn; Protein tyrosine kinase fyn; Proto oncogene tyrosine protein kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; Protooncogene Syn; SLK; Src like kinase; Src yes related novel gene; Src-like kinase; Src/yes related novel; Src/yes related novel gene; SYN; Tyrosine kinase p59fyn T; Tyrosine kinase p59fyn; T; Tyrosine-protein kinase Fyn.
Documentation
[1]. Rouer E. Les isoformes neuronales des tyrosines kinases Src, et al. Les isoformes neuronales des tyrosines kinases Src, Fyn et Lck : rôle particulier de la p56lckN dans la survie neuronale [Neuronal isoforms of Src, Fyn and Lck tyrosine kinases: A specific role for p56lckN in neuron protection]. C R Biol. 2010 Jan;333(1):1-10. French. [Content Brief]
[3]. Sharma S, et al. Mechanisms of disease-modifying effect of saracatinib (AZD0530), a Src/Fyn tyrosine kinase inhibitor, in the rat kainate model of temporal lobe epilepsy. Neurobiol Dis. 2021 Aug;156:105410. [Content Brief]
Powered by Bioz
