TREX1 Antibody (YA1732)
(Synonyms: AGS1; AGS5; CRV; DNase III; DRN3; HERNS; TREX1)TREX1 Antibody (YA1732) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TREX1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-F, IHC-P, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:100 | 1:50-1:200 |
Product Details
TREX1 Antibody (YA1732) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TREX1.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
Recombinant protein of human TREX1
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TREX1 is a Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability: its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation. Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation. Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair[1][2][3][4][5][6][7].
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Subcellular Localization
Nucleus; Cytoplasm, cytosol; Endoplasmic reticulum membrane; Peripheral membrane protein
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Expression
Tissue_specificity:Detected in thymus, spleen, liver, brain, heart, small intestine and colon -
Isoforms & Post-Translational Modification
Q9NSU2 has 3 isomers: Q9NSU2-3: 33212 Da (predicted); Q9NSU2-1: 38923 Da (predicted); Q9NSU2-2: 32276 Da (predicted).
Ubiquitinated, but not targeted to proteasomal degradation. Ubiquitination may be important for interaction with UBQLN1 -
Subunit
Homodimer (By similarity). Interacts (via proline-rich region) with TCERG1/CA150 (via the second WW domain) (By similarity).
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SwissProt ID
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Synonyms
AGS1; AGS5; CRV; DNase III; DRN3; HERNS; TREX1
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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