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Glutamine Synthetase Antibody (YA405)

(Synonyms: GLNA; GS; GLUL; GLNS; PIG43; PIG59; Glutamine synthetase)
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Based on 1 Customer Validation

Glutamine Synthetase Antibody (YA405) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutamine Synthetase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
  • Glutamine Synthetase Antibody (YA405) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Glutamine Synthetase Antibody (YA405) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutamine Synthetase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Glutamine Synthetase.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102955

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
    Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), Rat brain (lane 2, 15 μg), and Mouse liver (lane 3, 15 μg) using Glutamine Synthetase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
    Western blot analysis of extracts from Jurkat(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Glutamine Synthetase Antibody (HY-P80691) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Glutamine Synthetase is a Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia. Plays a key role in ammonium detoxification during erythropoiesis: the glutamine synthetase activity is required to remove ammonium generated by porphobilinogen deaminase (HMBS) during heme biosynthesis to prevent ammonium accumulation and oxidative stress. Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis. Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm, cytosol; Microsome; Mitochondrion; Cell membrane; Lipid-anchor

  • Expression


    Tissue_specificity:Expression in endothelial cells

    Induction:By glucocorticoids. Vitamin D and the Wnt signaling pathway inhibit its expression and activity (PubMed:18555765) . Glutamine synthase (GS) levels are regulated by a negative feedback mechanism where elevated glutamine levels stimulate GS ubiquitin-mediated proteasomal degradation (PubMed:26990986, PubMed:38579670)

  • Subunit

    Decamer; composed of two pentamers (PubMed:18005987). Interacts with PALMD (By similarity). Interacts with RHOJ (PubMed:30158707). Interacts with BEST2; this interaction tethers a fraction of GLUL to the membrane, causing a decrease of cytosolic glutamine synthase (GS) activity and inhibits the chloride channel activity of BEST2 by affecting the gating at the aperture in the absence of intracellular glutamate (PubMed:36289327)

  • SwissProt ID

    P15104

  • Gene ID
    2752 [NCBI]
  • Synonyms

    GLNA; GS; GLUL; GLNS; PIG43; PIG59; Glutamine synthetase

  • Research Field

    Neuroscience

Glutamine Synthetase Antibody (YA405) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), Rat brain (lane 2, 15 μg), and Mouse liver (lane 3, 15 μg) using Glutamine Synthetase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Glutamine Synthetase Antibody (HY-P80691) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Glutamine Synthetase Antibody (YA405) Product Image
    1/16
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
  • Glutamine Synthetase Antibody (YA405) Product Image
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), Rat brain (lane 2, 15 μg), and Mouse liver (lane 3, 15 μg) using Glutamine Synthetase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Glutamine Synthetase Antibody (HY-P80691) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA405)
  • Glutamine Synthetase Antibody (YA405) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), Rat brain (lane 2, 15 μg), and Mouse liver (lane 3, 15 μg) using Glutamine Synthetase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Glutamine Synthetase Antibody (YA405) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Glutamine Synthetase Antibody (HY-P80691) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Glutamine Synthetase Antibody (YA405) Product Image WB
  • Glutamine Synthetase Antibody (YA405) Product Image WB