CD248 Antibody (YA4508)(PBS only)

Cat. No.: HY-P84811A: Data Sheet User Guide for Antibodies
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CD248 Antibody (YA4508) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD248.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD248 Antibody (YA4508) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD248.

Host

Mouse

Molecular Weight

Predicted band size: 80.9 kDa;

Observed band size: 160 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9HCU0

Gene ID

57124 [NCBI]

Immunogen

Purified recombinant fragment of human CD248 (AA: extra 18-180) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Cell surface glycoprotein involved in various biological processes including angiogenesis, immune response modulation, and tissue remodeling and repair. Participates in pericyte proliferation through positive modulation of the PDGF receptor signaling pathway (PubMed:20484976). Acts as a scaffold for factor X, triggering allosteric changes and the spatial re-alignment of factor X with the TF-factor VIIa complex, thereby enhancing coagulation activation. Modulates the insulin signaling pathway by interacting with insulin receptor/INSR and by diminishing its capacity to be autophosphorylated in response to insulin. Also regulates LPS-induced inflammatory response in macrophages by favoring the production of proinflammatory cytokines. In human, negatively regulates T-cell proliferation compared with stromal cells where it increases proliferation (PubMed:21466550)

Subcellular Localization：Membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts. Strongly expressed in pericytes (PubMed:20484976) . Expressed on stromal cells and cells with lymphoid morphology such a T-cells (PubMed:21466550)

Isoforms & Post-Translational Modification：Q9HCU0 has 2 isomers: Q9HCU0-1: 80859 Da (predicted); Q9HCU0-2: 46241 Da (predicted).
O-glycosylated with sialylated oligosaccharides;May be N-glycosylated

Subunit：Interacts with PDGFRA; this interaction promotes PDGF receptor signaling pathway. Interacts with integrin beta-1/ITGB1.

Synonyms

TEM1; CD164L1

Documentation

User Guide for Antibodies (1077 KB)