N4BP1 Antibody (YA3286)

Cat. No.: HY-P83541: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

N4BP1 Antibody (YA3286) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to N4BP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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N4BP1 Antibody (YA3286) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

N4BP1 Antibody (YA3286) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to N4BP1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 99 kDa;

Observed band size: 99 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q6A037

Gene ID

80750 [NCBI]

Immunogen

Recombinant protein of mouse N4BP1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using N4BP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Potent suppressor of cytokine production that acts as a regulator of innate immune signaling and inflammation (PubMed:32971525). Acts as a key negative regulator of select cytokine and chemokine responses elicited by TRIF-independent Toll-like receptors (TLRs), thereby limiting inflammatory cytokine responses to minor insults (PubMed:32971525). In response to more threatening pathogens, cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation, thereby allowing production of inflammatory cytokines (PubMed:32971525). Acts as a restriction factor against some viruses: restricts viral replication by binding to mRNA viruses and mediating their degradation via its ribonuclease activity (By similarity). Also acts as an inhibitor of the E3 ubiquitin-protein ligase ITCH: acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (PubMed:17592138)

Subcellular Localization：Cytoplasm, cytosol; Nucleus; Nucleus, nucleolus; Nucleus, PML body

Subunit：Interacts with NEDD4 (PubMed:11717310). Interacts with ITCH (via WW domain 2) (PubMed:17592138)

RRID

AB_3105650

Database

Entrez Gene: 80750 Human

SwissProt: Q6A037 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

C81621; AI481586

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

N4BP1 Antibody (YA3286) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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N4BP1 Antibody (YA3286)

N4BP1 Antibody (YA3286) Featured Recommendations:

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Background

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N4BP1 Antibody (YA3286) Related Classifications

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