Copy Link Print Information

RASGRP1 Antibody (YA2546)

(Synonyms: V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII)
Customer Review

Based on 1 Customer Validation

RASGRP1 Antibody (YA2546) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RASGRP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • RASGRP1 Antibody (YA2546) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

RASGRP1 Antibody (YA2546) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RASGRP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 90 kDa
Species Reactivity Database

Entrez Gene: 10125 Human

SwissProt: O95267 Human

Immunogen

A synthetic peptide of human Rasgrp1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104895

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    RASGRP1 functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways. Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses. May also function in differentiation of other cell types[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Cytoplasm, cytosol; Cell membrane; Peripheral membrane protein; Golgi apparatus membrane; Peripheral membrane protein; Endoplasmic reticulum membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity:Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level) . Expressed in NK cells (at protein level) (PubMed:19933860)

  • Isoforms & Post-Translational Modification

    O95267 has 5 isomers: O95267-1: 90402 Da (predicted); O95267-2: 86625 Da (predicted); O95267-3: 68170 Da (predicted); O95267-4: 55100 Da (predicted); O95267-5: 62674 Da (predicted).

  • Subunit

    Homodimer (PubMed:23908768). Forms a signaling complex with DGKZ and HRAS (PubMed:11257115). Interacts with F-actin (PubMed:12839994). Interacts with SKAP1 (PubMed:17658605)

  • SwissProt ID

    O95267

  • Gene ID
    10125 [NCBI]
  • Synonyms

    V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII

  • Research Field

    Neuroscience

[1]. Roose JP, et al. A diacylglycerol-protein kinase C-RasGRP1 pathway directs Ras activation upon antigen receptor stimulation of T cells. Mol Cell Biol. 2005 Jun;25(11):4426-41. [Content Brief]

[2]. Iwig JS, et al. Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1. Elife. 2013 Jul 30;2:e00813. [Content Brief]

[3]. Salzer E, et al. RASGRP1 deficiency causes immunodeficiency with impaired cytoskeletal dynamics. Nat Immunol. 2016 Dec;17(12):1352-1360. [Content Brief]

[4]. Mao H, et al. RASGRP1 mutation in autoimmune lymphoproliferative syndrome-like disease. J Allergy Clin Immunol. 2018 Aug;142(2):595-604.e16. [Content Brief]

[5]. Ebinu JO, et al. RasGRP links T-cell receptor signaling to Ras. Blood. 2000 May 15;95(10):3199-203. [Content Brief]

[6]. Caloca MJ, et al. Vav mediates Ras stimulation by direct activation of the GDP/GTP exchange factor Ras GRP1. EMBO J. 2003 Jul 1;22(13):3326-36. [Content Brief]

[7]. Lee SH, et al. RasGRP1 is required for human NK cell function. J Immunol. 2009 Dec 15;183(12):7931-8. [Content Brief]

[8]. Bivona TG, et al. Phospholipase Cgamma activates Ras on the Golgi apparatus by means of RasGRP1. Nature. 2003 Aug 7;424(6949):694-8. [Content Brief]

View More

RASGRP1 Antibody (YA2546) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • RASGRP1 Antibody (YA2546) Product Image
    1/16
  • RASGRP1 Antibody (YA2546) Product Image