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Phospho-LAT (Tyr191) Antibody (YA1021)

(Synonyms: LAT; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38)
Customer Review

Based on 1 Customer Validation

Phospho-LAT (Tyr191) Antibody (YA1021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-LAT (Tyr191).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:30

Product Details

Description

Phospho-LAT (Tyr191) Antibody (YA1021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-LAT (Tyr191).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Phospho-LAT (Y191)

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3103392

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Western blot analysis was performed on extracts from 3T3 (lane 1, 15 μg), 3T3+PMA (lane 2, 15 μg), Hela (lane 3, 15 μg), Hela+Na3VO4 (lane 4, 15 μg), Jurkat (lane 5, 15 μg), and HepG2 (lane 6, 15 μg) using Phospho-LAT (Tyr191) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Immunohistochemical analysis of paraffin-embedded human placenta‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    LAT is required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules[1][2].

  • Subcellular Localization

    Cell membrane; Single-pass type III membrane protein

  • Expression


    Tissue_specificity:It is expressed in the thymus, T cells, NK cells, and mast cells, with lower expression levels in the spleen. It is present in T cells but not in B cells (at the protein level) .

  • Isoforms & Post-Translational Modification

    O43561 has 5 isomers: O43561-1: 27930 Da (predicted); O43561-2: 24985 Da (predicted); O43561-3: 28568 Da (predicted); O43561-4: 24829 Da (predicted); O43561-5: 27774 Da (predicted).
    Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes;Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation;'Lys-63'-linked ubiquitinated by TRAF6

  • Subunit

    When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK (By similarity). Interacts with FCGR1A. Interacts with GRB2, PLCG1 and THEMIS upon TCR activation in thymocytes (By similarity). Interacts with THEMIS2 (By similarity)

  • SwissProt ID

    O43561

  • Gene ID
    27040 [NCBI]
  • Synonyms

    LAT; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38

  • Research Field

    Immunology

Phospho-LAT (Tyr191) Antibody (YA1021) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 3T3 (lane 1, 15 μg), 3T3+PMA (lane 2, 15 μg), Hela (lane 3, 15 μg), Hela+Na3VO4 (lane 4, 15 μg), Jurkat (lane 5, 15 μg), and HepG2 (lane 6, 15 μg) using Phospho-LAT (Tyr191) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    1/16
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 3T3 (lane 1, 15 μg), 3T3+PMA (lane 2, 15 μg), Hela (lane 3, 15 μg), Hela+Na3VO4 (lane 4, 15 μg), Jurkat (lane 5, 15 μg), and HepG2 (lane 6, 15 μg) using Phospho-LAT (Tyr191) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Experimental Validation Results for Phospho-LAT (Tyr191) Antibody (YA1021)
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 3T3 (lane 1, 15 μg), 3T3+PMA (lane 2, 15 μg), Hela (lane 3, 15 μg), Hela+Na3VO4 (lane 4, 15 μg), Jurkat (lane 5, 15 μg), and HepG2 (lane 6, 15 μg) using Phospho-LAT (Tyr191) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta‌ tissue using Phospho-LAT (Tyr191) antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81297, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma‌ tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using Phospho-LAT (Tyr191) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81297, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image WB
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image IHC-P
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image IHC-P
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image IHC-P
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image IHC-P
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image IHC-P
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image IHC-P
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image mIHC
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image mIHC
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image mIHC
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image mIHC
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image mIHC
  • Phospho-LAT (Tyr191) Antibody (YA1021) Product Image mIHC