CD209 Antibody (YA4510)(PBS only)

Cat. No.: HY-P84813A: Data Sheet User Guide for Antibodies
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CD209 Antibody (YA4510) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD209.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD209 Antibody (YA4510) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD209.

Host

Mouse

Molecular Weight

Predicted band size: 46 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NNX6

Gene ID

30835 [NCBI]

Immunogen

Purified recombinant fragment of human CD209 (AA: extra 270-404) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response; On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells; (Microbial infection) Acts as an attachment receptor for HIV-1 and HIV-2; (Microbial infection) Acts as an attachment receptor for Ebolavirus; (Microbial infection) Acts as an attachment receptor for Cytomegalovirus; (Microbial infection) Acts as an attachment receptor for HCV; (Microbial infection) Acts as an attachment receptor for Dengue virus; (Microbial infection) Acts as an attachment receptor for Measles virus; (Microbial infection) Acts as an attachment receptor for Herpes simplex virus 1; (Microbial infection) Acts as an attachment receptor for Influenzavirus A; (Microbial infection) Acts as an attachment receptor for SARS-CoV; (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus; (Microbial infection) Acts as an attachment receptor for Lassa virus (PubMed:23966408). Acts as an attachment receptor for Marburg virusn; (Microbial infection) Acts as an attachment receptor for Respiratory syncytial virus; (Microbial infection) Acts as an attachment receptor for Rift valley fever virus and uukuniemi virus; (Microbial infection) Acts as an attachment receptor for West-nile virus; (Microbial infection) Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of bacterial pathogen antigens, including Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA (PubMed:16379498). Recognition of M.tuberculosis by dendritic cells occurs partially via this molecule (PubMed:16092920, PubMed:21203928)

Subcellular Localization：Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Secreted; Secreted; Secreted; Secreted; Secreted; Secreted; Secreted

Expression：
Tissue_specificity:It is mainly expressed in dendritic cells and the tissues in which dendritic cells reside. It is also found in placental macrophages, placental vascular endothelial cells, peripheral blood monocytes, and THP-1 monocytes.

Isoforms & Post-Translational Modification：Q9NNX6 has 12 isomers: Q9NNX6-1: 45775 Da (predicted); Q9NNX6-2: 45031 Da (predicted); Q9NNX6-3: 35236 Da (predicted); Q9NNX6-4: 18646 Da (predicted); Q9NNX6-5: 45571 Da (predicted); Q9NNX6-6: 43330 Da (predicted); Q9NNX6-7: 41009 Da (predicted); Q9NNX6-8: 30427 Da (predicted); Q9NNX6-9: 4046 Da (predicted); Q9NNX6-10: 43126 Da (predicted); Q9NNX6-11: 37844 Da (predicted); Q9NNX6-12: 33874 Da (predicted).

Subunit：Homotetramer. Interacts with C1QBP; the interaction is indicative for a C1q:C1QBP:CD209 signaling complex. Interacts with ICAM2 and ICAM3 by binding to mannose-like carbohydrates. Interacts (via C-type lectin domain) with CEACAM1 (via Lewis X moieties); this interaction is regulated by the glycosylation pattern of CEACAM1 on cell types and regulates contact between dendritic cells and neutrophils (PubMed:16246332)

Synonyms

CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1

Documentation

User Guide for Antibodies (1077 KB)