Copy Link Print Information

PD-1 Antibody(YA3401)

(Synonyms: Programmed cell death protein 1; CD279; CD279 antigen; hPD 1; hPD-1; hSLE1; PD 1; PD1; PDCD 1; PDCD1; PDCD1_HUMAN; Programmed cell death 1; Protein PD 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2.)
4 Cited Publications
Customer Review

Based on 4 publication(s) in Google Scholar

PD-1 Antibody(YA3401) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PD-1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • PD-1 Antibody(YA3401) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) PD-1 Antibody(YA3401)

More

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:50-1:200 1:100-1:500 1:50-1:100

Product Details

Description

PD-1 Antibody(YA3401) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PD-1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 32 kDa,47-55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Species Reactivity Database

Entrez Gene: 5133 Human

SwissProt: Q15116 Human

Immunogen

Recombinant protein within Human PD1 aa 1-200 / 288.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3676598

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PD-1 Antibody(YA3401)
    Western blot analysis of extracts from Jurkat(lane 2(20μg), Molt-4(lane 3(20μg), THP-1(lane 4(20μg) and K562(lane 5(20μg) using PD-1 Antibody (HY-P81169A). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue(negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

  • Function

    CD279 (PD-1, PDCD1) is a classic immune checkpoint receptor primarily expressed on activated T cells, exhausted T cells, Tregs, and a subset of NK cells. By binding to PD-L1/PD-L2 and inhibiting TCR signaling, it suppresses T-cell proliferation and cytotoxic function; it serves as a key target in research concerning tumor immune escape, T-cell exhaustion, and immunotherapy.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Subunit

    Monomer. Interacts with CD274/PDCD1L1. Interacts with FBXO38; leading to ubiquitination and degradation of PDCD1 by the proteasome

  • SwissProt ID

    Q15116

  • Gene ID
    5133 [NCBI]
  • Synonyms

    Programmed cell death protein 1; CD279; CD279 antigen; hPD 1; hPD-1; hSLE1; PD 1; PD1; PDCD 1; PDCD1; PDCD1_HUMAN; Programmed cell death 1; Protein PD 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2.

[1]. Fife BT, et al. The role of the PD-1 pathway in autoimmunity and peripheral tolerance. Ann N Y Acad Sci. 2011 Jan;1217:45-59. [Content Brief]

[2]. Xiao X, et al. ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nat Commun. 2023 May 19;14(1):2859. [Content Brief]

[3]. Berger KN, et al. PD-1 pathway and its clinical application: A 20year journey after discovery of the complete human PD-1 gene. Gene. 2018 Jan 5;638:20-25. [Content Brief]

[4]. Topalian SL, et al. Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N Engl J Med. 2012 Jun 28;366(26):2443-54. [Content Brief]

[5]. Robert C, et al. Anti-programmed-death-receptor-1 treatment with pembrolizumab in ipilimumab-refractory advanced melanoma: a randomised dose-comparison cohort of a phase 1 trial. Lancet. 2014 Sep 20;384(9948):1109-17. [Content Brief]

[6]. Robert C, et al. Nivolumab in previously untreated melanoma without BRAF mutation. N Engl J Med. 2015 Jan 22;372(4):320-30. [Content Brief]

View More

PD-1 Antibody(YA3401) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20μg), Molt-4(lane 3(20μg), THP-1(lane 4(20μg) and K562(lane 5(20μg) using PD-1 Antibody (HY-P81169A). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue(negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PD-1 Antibody(YA3401) Product Image
    1/16
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • PD-1 Antibody(YA3401) Product Image
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20μg), Molt-4(lane 3(20μg), THP-1(lane 4(20μg) and K562(lane 5(20μg) using PD-1 Antibody (HY-P81169A). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue(negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • Experimental Validation Results for PD-1 Antibody(YA3401)
  • PD-1 Antibody(YA3401) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20μg), Molt-4(lane 3(20μg), THP-1(lane 4(20μg) and K562(lane 5(20μg) using PD-1 Antibody (HY-P81169A). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • PD-1 Antibody(YA3401) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue(negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PD-1 Antibody(YA3401) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PD-1 Antibody(YA3401) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PD-1 Antibody(YA3401) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PD-1 Antibody(YA3401) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PD-1 Antibody(YA3401) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PD-1 Antibody(YA3401) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PD-1 Antibody(YA3401) Product Image WB
  • PD-1 Antibody(YA3401) Product Image IHC-P
  • PD-1 Antibody(YA3401) Product Image IHC-P
  • PD-1 Antibody(YA3401) Product Image IHC-P
  • PD-1 Antibody(YA3401) Product Image IHC-P
  • PD-1 Antibody(YA3401) Product Image IHC-P
  • PD-1 Antibody(YA3401) Product Image IHC-P
  • PD-1 Antibody(YA3401) Product Image IHC-P