JMJD3 Antibody
(Synonyms: JMJD3, KIAA0346, KDM6B, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, [histone H3]-trimethyl-L-lysine(27) demethylase 6B)
Based on 1 Customer Validation
JMJD3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to JMJD3.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, ICC/IF
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-200 | 1:50-200 |
Product Details
JMJD3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to JMJD3.
-
Host Rabbit
-
Clonality Polyclonal
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 177 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 176 kDa
Purified recombinant protein of human JMJD3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
JMJD3 is a Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (By similarity)[1][2][3][4].
-
Subcellular Localization
Nucleus
-
Expression
Induction: By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. -
Isoforms & Post-Translational Modification
JMJD3 has 2 isoforms, O15054-2: amino acid length is 1643, molecular weight is 176632 Da (predicted); O15054-1: amino acid length is 1682, molecular weight is 180702 Da (predicted).
-
Subunit
Interacts with TLE1 (By similarity).
-
SwissProt ID
-
Synonyms
JMJD3, KIAA0346, KDM6B, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, [histone H3]-trimethyl-L-lysine(27) demethylase 6B
Documentation
[1]. Agger K, et al. UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature. 2007 Oct 11;449(7163):731-4. [Content Brief]
[2]. De Santa F, et al. The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing. Cell. 2007 Sep 21;130(6):1083-94. [Content Brief]
[3]. Lan F, et al. A histone H3 lysine 27 demethylase regulates animal posterior development. Nature. 2007 Oct 11;449(7163):689-94. [Content Brief]
[4]. Hong S, et al. Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18439-44. [Content Brief]
Powered by Bioz
