ATP6AP1 Antibody (YA4304)(PBS only)
(Synonyms: 16A; CF2; Ac45; XAP3; XAP-3; ATP6S1; VATPS1; ATP6IP1)ATP6AP1 Antibody (YA4304) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP6AP1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, FC, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:400 | 1:10000 |
Product Details
ATP6AP1 Antibody (YA4304) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP6AP1.
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Host Mouse
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 90-130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 52 kDa
Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ATP6AP1 is an Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex (Probable). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. In islets of Langerhans cells, may regulate the acidification of dense-core secretory granules[1][2][3].
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Subcellular Localization
Endoplasmic reticulum membrane; Single-pass type I membrane protein; Endoplasmic reticulum-Golgi intermediate compartment membrane; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type I membrane protein; Cytoplasmic vesicle, clathrin-coated vesicle membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:widely expressed, with highest levels in brain and lowest in liver and duodenum -
Subunit
Accessory component of the multisubunit proton-transporting vacuolar (V)-ATPase protein pump (PubMed:33065002). Interacts (via N-terminus) with ATP6AP2 (via N-terminus) (PubMed:29127204, PubMed:33065002). Interacts with RNASEK (PubMed:26212330). Interacts with TMEM106B (via C-terminus) (PubMed:28728022)
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SwissProt ID
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Synonyms
16A; CF2; Ac45; XAP3; XAP-3; ATP6S1; VATPS1; ATP6IP1
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