ATP6AP1 Antibody (YA4304)(PBS only)

Cat. No.: HY-P84607A: Data Sheet User Guide for Antibodies
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ATP6AP1 Antibody (YA4304) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP6AP1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ATP6AP1 Antibody (YA4304) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP6AP1.

Host

Mouse

Molecular Weight

Predicted band size: 52 kDa;

Observed band size: 90-130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q15904

Gene ID

537 [NCBI]

Immunogen

Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles (PubMed:33065002). Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity (PubMed:27231034). Involved in membrane trafficking and Ca(2+)-dependent membrane fusion (PubMed:27231034). May play a role in the assembly of the V-type ATPase complex (Probable). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). In islets of Langerhans cells, may regulate the acidification of dense-core secretory granules (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass type I membrane protein; Endoplasmic reticulum-Golgi intermediate compartment membrane; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type I membrane protein; Cytoplasmic vesicle, clathrin-coated vesicle membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:widely expressed, with highest levels in brain and lowest in liver and duodenum

Subunit：Accessory component of the multisubunit proton-transporting vacuolar (V)-ATPase protein pump (PubMed:33065002). Interacts (via N-terminus) with ATP6AP2 (via N-terminus) (PubMed:29127204, PubMed:33065002). Interacts with RNASEK (PubMed:26212330). Interacts with TMEM106B (via C-terminus) (PubMed:28728022)

Synonyms

16A; CF2; Ac45; XAP3; XAP-3; ATP6S1; VATPS1; ATP6IP1

Documentation

User Guide for Antibodies (1077 KB)