PPCEL Antibody (YA3175)(PBS only)

Cat. No.: HY-P83430A: Data Sheet User Guide for Antibodies
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PPCEL Antibody (YA3175) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPCEL.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PPCEL Antibody (YA3175) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPCEL.

Host

Rabbit

Molecular Weight

Predicted band size: 84 kDa;

Observed band size: 72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Hamster

SwissProt ID

Q4J6C6

Gene ID

9581 [NCBI]

Immunogen

Recombinant protein of human PPCEL/PREPL aa451-651.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine peptidase whose precise substrate specificity remains unclear (PubMed:16143824, PubMed:16385448, PubMed:28726805). Does not cleave peptides after a arginine or lysine residue (PubMed:16143824). Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex (PubMed:23321636). May play a role in the regulation of synaptic vesicle exocytosis (PubMed:24610330)

Subcellular Localization：Cytoplasm, cytosol; Golgi apparatus, trans-Golgi network; Cytoplasm, cytoskeleton; Golgi apparatus; Nucleus

Expression：
Tissue_specificity:Expressed in pyramidal neurons of the temporal cortex and neocortex (at protein level) (PubMed:23485813) . Widely expressed (PubMed:15913950, PubMed:16385448) . Expressed at higher level in brain, skeletal muscle, heart and kidney (PubMed:15913950, PubMed:16385448) . Expressed at the endplates in the neuromuscular junction (PubMed:24610330)

Isoforms & Post-Translational Modification：Q4J6C6 has 4 isomers: Q4J6C6-1: 83927 Da (predicted); Q4J6C6-2: 76352 Da (predicted); Q4J6C6-3: 76757 Da (predicted); Q4J6C6-4: 73352 Da (predicted).

Subunit：Homodimer (PubMed:16143824). Interacts with the AP-1 complex (PubMed:23321636)

Research Field

Synonyms

CMS22; Prolylendopeptidase-like

Documentation

User Guide for Antibodies (1077 KB)