TTF-1 Antibody (YA2297)

Cat. No.: HY-P82552: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

TTF-1 Antibody (YA2297) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TTF-1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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TTF-1 Antibody (YA2297) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

TTF-1 Antibody (YA2297) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TTF-1.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 39 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Hamster

SwissProt ID

P43699

Gene ID

7080 [NCBI]

Immunogen

A synthetic peptide of human TTF1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293 (lane 2, 15 μg), DU145 (lane 3, 15 μg), COLO320 (lane 4, 15 μg), Rat lung (lane 5, 15 μg), Mouse lung (lane 6, 15 μg) using Thyroid Transcription Factor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:thyroid and lungs

Isoforms & Post-Translational Modification：P43699 has 2 isomers: P43699-1: 38596 Da (predicted); P43699-3: 41737 Da (predicted).
Phosphorylated on serine residues by STK3/MST2

Subunit：Interacts with WWTR1

Database

Entrez Gene: 7080 Human ; 21869 Mouse ;

SwissProt: P43699 Human ; P50220 Mouse ; P23441 Rat

OMIM: 118700 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

NKX2-1; NKX2A; TITF1; TTF1; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

TTF-1 Antibody (YA2297) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TTF-1 Antibody (YA2297)

TTF-1 Antibody (YA2297) Featured Recommendations:

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Background

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TTF-1 Antibody (YA2297) Related Classifications

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