MST4 Antibody (YA3253)
(Synonyms: MASK; MST4)
Based on 1 Customer Validation
MST4 Antibody (YA3253) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MST4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:5000 | 1:50-1:100 | 1:200-1:500 | 1:100-1:1000 | 1:20-1:50 |
Product Details
MST4 Antibody (YA3253) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MST4.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 47 kDa
A synthetic peptide of human MST4 aa1-13.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using MST4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83508, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta using MST4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83508, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human melanoma using MST4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83508, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human head and neck cancer using MST4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83508, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cervical cancer using MST4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83508, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human ovarian cancer using MST4 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83508, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
Hippo (MST) MST4 is a serine/threonine kinase of the germinal center kinase III family that regulates cell growth, apoptosis, polarity, migration, and transformation-related signaling programs[1][2]. Unlike the canonical Hippo kinases MST1 and MST2, which function as core upstream components of the Hippo tumor-suppressor cascade, MST4 primarily acts through distinct signaling modules including ERK-dependent pathways and Golgi-associated regulatory networks, highlighting functional divergence within the MST kinase family[3][4]. Mechanistically, MST4 localizes to the Golgi apparatus, where interaction with the Golgi matrix protein GM130 contributes to the control of cell polarity and migration. Through modulation of ERK signaling, MST4 promotes cellular proliferation and transformation-related phenotypes in experimental systems[2][5]. In disease models, elevated MST4 expression has been associated with aggressive behavior in multiple cancers, including prostate cancer and hepatocellular carcinoma, where MST4 enhances proliferation, invasion, epithelial-mesenchymal transition, and metastatic potential[5][6]. Beyond tumor biology, MST4 also participates in innate immune regulation by phosphorylating TRAF6 and suppressing excessive Toll-like receptor-mediated inflammatory responses, indicating broader functions in tissue homeostasis and host defense[7]. Compared with related isoforms MST1 and MST2, which are central Hippo pathway regulators, MST4 is more strongly linked to Golgi-associated signaling, ERK activation, and context-dependent regulation of cancer progression and inflammation[3][7]. Small-molecule MST4 inhibitors have been reported as experimental tools for mechanistic studies and therapeutic target validation in disease models[8].
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Subcellular Localization
Cytoplasm; Golgi apparatus
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Isoforms & Post-Translational Modification
Q9P289 has 3 isomers: Q9P289-1: 46529 Da (predicted); Q9P289-2: 37770 Da (predicted); Q9P289-3: 39658 Da (predicted).
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Subunit
Homodimer (PubMed:20730082). Interacts with PDCD10 (PubMed:17360971, PubMed:19370760, PubMed:20332113). Interacts with GOLGA2 (PubMed:15037601, PubMed:20332113). Interacts with CTTNBP2NL (PubMed:18782753). Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner (PubMed:27807006). Interacts with PDCD10; this interaction is required for the association of STK26 with RIPOR1 (PubMed:27807006). Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26 (PubMed:18782753)
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SwissProt ID
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Synonyms
MASK; MST4
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Research Field
Cell Biology
Documentation
References
[1]. Lee MJ, et al. Time to HIV rebound after infusion of long-acting broadly neutralising antibodies 3BNC117-LS and 10-1074-LS and analytical treatment interruption (the RIO trial): a double-blind, randomised, placebo-controlled trial. Lancet HIV. 2026 May 27:S2352-3018(26)00059-7. [Content Brief]
[2]. Bosc DG, et al. HER2/Neu-mediated activation of the ETS transcription factor ER81 and its target gene MMP-1. Oncogene. 2001 Sep 27;20(43):6215-24. [Content Brief]
[3]. Wrighton KH. Cell migration: EMT promotes contact inhibition of locomotion. Nat Rev Mol Cell Biol. 2015 Sep;16(9):518. doi: 10.1038/nrm4045. Epub 2015 Aug 12. PMID: 26265408. et al. Cell migration: EMT promotes contact inhibition of locomotion. Nat Rev Mol Cell Biol. 2015 Sep;16(9):518. [Content Brief]
[4]. Shadel GS, et al. Mitochondrial ROS signaling in organismal homeostasis. Cell. 2015 Oct 22;163(3):560-9. [Content Brief]
[5]. Villemure JF, et al. MSH2-deficient human cells exhibit a defect in the accurate repair of plasmid DNA double-strand breaks by homologous recombination. Cancer Res. 2003;63(12):3334-9. [Content Brief]
[6]. Lin ZH, et al. MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway. Int J Oncol. 2014 Aug;45(2):629-40. [Content Brief]
[7]. Shi Z, et al. MST kinases in innate immune signaling. Cell Stress. 2018;2(1):4-13.
[8]. Ali SR, et al. Nerve Density and Neuronal Biomarkers in Cancer. Cancers (Basel). 2022 Oct 1;14(19):4817. [Content Brief]
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