Fibulin 1 Antibody (YA2831)

製品番号: HY-P83086: データシート SDS COA User Guide for Antibodies
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Fibulin 1 Antibody (YA2831) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibulin 1.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$78	在庫あり	Estimated Time of Arrival: December 31
50 μL	$198	在庫あり	Estimated Time of Arrival: December 31
100 μL	$312	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

Fibulin 1 Antibody (YA2831) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibulin 1.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 77 kDa;

Observed band size: 77 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P23142

Gene ID

2192 [NCBI]

Immunogen

Recombinant protein of human Fibulin 1 266—402.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A549(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using Fibulin 1 Antibody (HY-P83086) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP

Subcellular Localization：Secreted, extracellular space, extracellular matrix

Expression：
Tissue_specificity:Isoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells

Induction:Expression increased by estrogen in ovarian cancer cells

Isoforms & Post-Translational Modification：P23142 has 4 isomers: P23142-1: 77214 Da (predicted); P23142-2: 61552 Da (predicted); P23142-3: 65443 Da (predicted); P23142-4: 74434 Da (predicted).

Subunit：Homomultimerizes and interacts with various extracellular matrix components such as FN1, LAMA1, LAMA2, NID, ACAN, CSPG2 and type IV collagen. Also interacts with APP and FGB. Interacts with FBLN7 (By similarity). Interacts with CCN3 (PubMed:9927660)

RRID

AB_3105202

Database

Entrez Gene: 2192 Human ; 14114 Mouse ; 315191 Rat

SwissProt: P23142 Human ; Q08879 Mouse ;

OMIM: 135820 Human

Research Field

Cardiovascular

Synonyms

FBLN; FIBL1

ドキュメンテーション

Select Batch:

データシート (258 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/mcf7-cell.html [Content Brief]