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Fibulin 1 Antibody (YA2831)

(Synonyms: FBLN; FIBL1)
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Based on 1 Customer Validation

Fibulin 1 Antibody (YA2831) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibulin 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Fibulin 1 Antibody (YA2831)
  • Fibulin 1 Antibody (YA2831) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Fibulin 1 Antibody (YA2831) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibulin 1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 77 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 77 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human Fibulin 1 266—402.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105202

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Fibulin 1 Antibody (YA2831)
    Western blot analysis of extracts from A549(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using Fibulin 1 Antibody (HY-P83086) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Fibulin 1 is an Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:Isoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells

    Induction:Expression increased by estrogen in ovarian cancer cells

  • Isoforms & Post-Translational Modification

    P23142 has 4 isomers: P23142-1: 77214 Da (predicted); P23142-2: 61552 Da (predicted); P23142-3: 65443 Da (predicted); P23142-4: 74434 Da (predicted).

  • Subunit

    Homomultimerizes and interacts with various extracellular matrix components such as FN1, LAMA1, LAMA2, NID, ACAN, CSPG2 and type IV collagen. Also interacts with APP and FGB. Interacts with FBLN7 (By similarity). Interacts with CCN3 (PubMed:9927660)

  • SwissProt ID

    P23142

  • Gene ID
    2192 [NCBI]
  • Synonyms

    FBLN; FIBL1

  • Research Field

    Cardiovascular

Fibulin 1 Antibody (YA2831) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using Fibulin 1 Antibody (HY-P83086) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Fibulin 1 Antibody (YA2831) Product Image
    1/16
  • Experimental Validation Results for Fibulin 1 Antibody (YA2831)
  • Fibulin 1 Antibody (YA2831) Product Image
  • Experimental Validation Results for Fibulin 1 Antibody (YA2831)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using Fibulin 1 Antibody (HY-P83086) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Fibulin 1 Antibody (YA2831)
  • Fibulin 1 Antibody (YA2831) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using Fibulin 1 Antibody (HY-P83086) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Fibulin 1 Antibody (YA2831) Product Image WB