Clathrin Heavy Chain Antibody (YA6273)
Based on 1 Customer Validation
Clathrin Heavy Chain Antibody (YA6273) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Clathrin Heavy Chain.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:50-200 | 1:500-1:2000 | 1:200-1000 | 1:5000-20000 |
Product Details
Clathrin Heavy Chain Antibody (YA6273) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Clathrin Heavy Chain.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 180 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 192 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Clathrin Heavy Chain is a Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice promoting uncoating[1][2][3][4][5].
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Subcellular Localization
Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side; Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side; Melanosome; Cytoplasm, cytoskeleton, spindle
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Isoforms & Post-Translational Modification
Q00610 has 2 isomers: Q00610-1: 191615 Da (predicted); Q00610-2: 187890 Da (predicted).
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Subunit
Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat (PubMed:16968737).
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SwissProt ID
Documentation
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