TRIM72 Antibody (YA9954)
(Synonyms: TRIM72; Mg53 antibody; Mitsugumin-53 antibody; Tripartite motif containing 72 antibody)TRIM72 Antibody (YA9954) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM72.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:50-1:200 |
Product Details
TRIM72 Antibody (YA9954) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM72.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 53 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 53 kDa
Recombinant protein with Human TRIM72 aa 50-150 / 477.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TRIM72 is a Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3 (By similarity). Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site (By similarity). This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation (By similarity). Probably acts upstream of the Ca(2+)-dependent membrane resealing process (By similarity). Required for transport of DYSF to sites of cell injury during repair patch formation (By similarity). Regulates membrane budding and exocytosis (By similarity). May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity)[1].
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Subcellular Localization
Cell membrane, sarcolemma; Cytoplasmic vesicle membrane
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Isoforms & Post-Translational Modification
TRIM72 has 2 isoforms, Q6ZMU5-1: amino acid length is 477, molecular weight is 52731 Da (predicted); Q6ZMU5-2: amino acid length is 269, molecular weight is 29501 Da (predicted).Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
TRIM72; Mg53 antibody; Mitsugumin-53 antibody; Tripartite motif containing 72 antibody
Documentation
References
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