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CD14 Antibody (YA6165)

(Synonyms: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14; [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form])
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CD14 Antibody (YA6165) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD14.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • CD14 Antibody (YA6165) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
IF Info
 
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000

Product Details

Description

CD14 Antibody (YA6165) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD14.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    CD14 is an important innate immune pattern recognition receptor, primarily serving as a component of the lipopolysaccharide (LPS) receptor complex. It plays a crucial role in recognizing Gram-negative bacteria and their products, and in activating inflammatory responses. CD14 itself lacks a transmembrane signal transduction structure; therefore, it typically functions as a co-receptor in synergy with TLR4 and MD-2.

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor; Secreted; Membrane raft; Golgi apparatus

  • Expression


    Tissue_specificity:Protein levels were detected on macrophages (PubMed: 1698311) . It is strongly expressed on the surface of monocytes and weakly expressed on the surface of granulocytes; it is also expressed in macrophages of most tissues.

    Induction:The expression in monocytes is highly induced by 27-hydroxycholesterol, priming monocytes/macrophages such that LPS-mediated inflammatory reaction is accelerated. Secretion of soluble CD14 is also enhanced

  • Subunit

    Interacts with LPS-bound LPB. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4.

  • SwissProt ID

    P08571

  • Gene ID
    929 [NCBI]
  • Synonyms

    Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14; [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form]

References

[1]. Wright SD, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990 Sep 21;249(4975):1431-3. [Content Brief]

[2]. Kelley SL, et al. The crystal structure of human soluble CD14 reveals a bent solenoid with a hydrophobic amino-terminal pocket. J Immunol. 2013 Feb 1;190(3):1304-11. [Content Brief]

[3]. Tsukamoto H, et al. Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD14-expressing cells. Int Immunol. 2010 Apr;22(4):271-80. [Content Brief]

[4]. Murakami K, et al. Follistatin-related protein/follistatin-like 1 evokes an innate immune response via CD14 and toll-like receptor 4. FEBS Lett. 2012 Feb 17;586(4):319-24. [Content Brief]

[5]. Haziot A, et al. Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice. Immunity. 1996 Apr;4(4):407-14. [Content Brief]

[6]. Triantafilou M, et al. Membrane sorting of toll-like receptor (TLR)-2/6 and TLR2/1 heterodimers at the cell surface determines heterotypic associations with CD36 and intracellular targeting. J Biol Chem. 2006 Oct 13;281(41):31002-11. [Content Brief]

[7]. Estruch M, et al. CD14 and TLR4 mediate cytokine release promoted by electronegative LDL in monocytes. Atherosclerosis. 2013 Aug;229(2):356-62. [Content Brief]

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CD14 Antibody (YA6165) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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