ULK3 Antibody (YA4337)(PBS only)

Cat. No.: HY-P84640A: Data Sheet User Guide for Antibodies
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ULK3 Antibody (YA4337) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ULK3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ULK3 Antibody (YA4337) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ULK3.

Host

Mouse

Molecular Weight

Predicted band size: 53 kDa;

Observed band size: 53 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q6PHR2

Gene ID

25989 [NCBI]

Immunogen

Purified recombinant fragment of human ULK3 aa 435-468(KLH).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Widely expressed. Highest concentration is found in the fetal brain. In human tissues, high concentrations are found in the brain, liver, and kidneys; moderate concentrations in the testes and adrenal glands; and low concentrations in the heart, lungs, stomach, thymus, prostate, and placenta. In the brain, the hippocampus shows the highest expression, with high concentrations also detected in the cerebellum, olfactory bulb, and optic nerve. The spinal cord shows the lowest concentration in the central nervous system.

Induction:Up-regulated during senescence

Isoforms & Post-Translational Modification：Q6PHR2 has 4 isomers: Q6PHR2-1: 53444 Da (predicted); Q6PHR2-2: 24402 Da (predicted); Q6PHR2-3: 53217 Da (predicted); Q6PHR2-4: 54997 Da (predicted).
Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU

Subunit：Interacts (via protein kinase domain) with SUFU

Synonyms

Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3

Documentation

User Guide for Antibodies (1077 KB)