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ADK Antibody (YA2689)

(Synonyms: ADK; Adenosine kinase; AK; Adenosine 5'-phosphotransferase)
Customer Review

Based on 1 Customer Validation

ADK Antibody (YA2689) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ADK.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • ADK Antibody (YA2689) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

ADK Antibody (YA2689) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ADK.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 41 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human ADK aa17-362.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105063

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ADK Antibody (YA2689)
    Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg), C6 (lane 2, 15 μg), Hela (lane 3, 15 μg), A549 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and U-87MG (lane 6, 15 μg) using ADK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ADK Antibody (YA2689)
    Western blot analysis of extracts from Hela(lane 2(20ug) ，A549(lane 3(20ug) and HL-60(lane 4(20ug) using ADK Antibody (HY-P82944) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADK Antibody (YA2689)
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using ADK Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ADK Antibody (YA2689)
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using ADK Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    ADK catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Widely expressed. Highest level in placenta, liver, muscle and kidney

  • Isoforms & Post-Translational Modification

    P55263 has 4 isomers: P55263-1: 40545 Da (predicted); P55263-2: 38703 Da (predicted); P55263-3: 34084 Da (predicted); P55263-4: 36580 Da (predicted).

  • Subunit

    Monomer

  • SwissProt ID

    P55263

  • Gene ID
    132 [NCBI]
  • Synonyms

    ADK; Adenosine kinase; AK; Adenosine 5'-phosphotransferase

  • Research Field

    Signal Transduction

ADK Antibody (YA2689) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg), C6 (lane 2, 15 μg), Hela (lane 3, 15 μg), A549 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and U-87MG (lane 6, 15 μg) using ADK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) ，A549(lane 3(20ug) and HL-60(lane 4(20ug) using ADK Antibody (HY-P82944) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using ADK Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using ADK Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ADK Antibody (YA2689) Product Image
    1/16
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • ADK Antibody (YA2689) Product Image
  • Experimental Validation Results for ADK Antibody (YA2689)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg), C6 (lane 2, 15 μg), Hela (lane 3, 15 μg), A549 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and U-87MG (lane 6, 15 μg) using ADK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ADK Antibody (YA2689)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) ，A549(lane 3(20ug) and HL-60(lane 4(20ug) using ADK Antibody (HY-P82944) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADK Antibody (YA2689)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using ADK Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ADK Antibody (YA2689)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using ADK Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • Experimental Validation Results for ADK Antibody (YA2689)
  • ADK Antibody (YA2689) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from RAW264.7 (lane 1, 15 μg), C6 (lane 2, 15 μg), Hela (lane 3, 15 μg), A549 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and U-87MG (lane 6, 15 μg) using ADK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ADK Antibody (YA2689) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) ，A549(lane 3(20ug) and HL-60(lane 4(20ug) using ADK Antibody (HY-P82944) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ADK Antibody (YA2689) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using ADK Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ADK Antibody (YA2689) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using ADK Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ADK Antibody (YA2689) Product Image WB
  • ADK Antibody (YA2689) Product Image WB
  • ADK Antibody (YA2689) Product Image IHC-P
  • ADK Antibody (YA2689) Product Image IHC-P