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PLK1 Antibody (YA843)

(Synonyms: PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

PLK1 Antibody (YA843) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PLK1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • PLK1 Antibody (YA843) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

PLK1 Antibody (YA843) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PLK1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PLK1 aa455-474.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103009

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PLK1 Antibody (YA843)
    Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug)using PLK1 Antibody (HY-P80972) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PLK1 Antibody (YA843)
    Immunocytochemistry analysis of Hela cells labeling PLK1 with PLK1 Antibody (HY-P80972) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PLK1 Antibody (HY-P80972) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PLK1 Antibody (YA843)
    Immunocytochemistry analysis of Hela cells labeling PLK1 with PLK1 Antibody (HY-P80972) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PLK1 Antibody (HY-P80972) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PLK1 is a serine/threonine Polo-like kinase that orchestrates cell division through mitotic entry, spindle-pole functions, and cytokinesis[1]. Mechanistically, mammalian PLK1 controls centrosome maturation, spindle assembly, and microtubule attachment to kinetochores in early mitosis[2]. This mitotic role makes PLK1 relevant to cancer biology, because dysfunction may promote cancerous transformation and drive progression, and overexpression occurs in diverse human cancers with poor prognosis[3]. Compared with related isoforms, PLK1-PLK4 participate in cell-cycle processes, but reported functional emphasis differs: PLK2 and PLK4 regulate centriole duplication, PLK3 regulates DNA replication, and PLK1 regulates centrosome separation and maturation[4]. For experimental applications, PLK1 inhibition provides a direct way to perturb mitosis, because BI 2536 inhibits mammalian PLK1 at low nanomolar concentrations, induces mitotic arrest and apoptosis in human cancer cell lines, and inhibits human tumor xenograft growth in nude mice[5].

  • Subcellular Localization

    Nucleus; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Midbody

  • Expression


    Tissue_specificity:placenta and colon

    Induction:By growth-stimulating agents

  • Subunit

    Interacts with CEP170 (PubMed:15616186). Interacts with EVI5 (PubMed:16439210). Interacts with FAM29A (PubMed:19029337). Interacts with SLX4/BTBD12 (PubMed:19596235). Interacts with TTDN1 (PubMed:17310276). Interacts (via POLO-box domain) with the phosphorylated form of BUB1, CDC25C and CENPU (PubMed:14532005, PubMed:16760428, PubMed:17307877, PubMed:19597481). Interacts with KIF2A (PubMed:19351716). Interacts with CYLD (PubMed:17495026). Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGO2 (PubMed:21402792). Interacts with BIRC6/bruce (PubMed:18329369). Interacts with CDK1-phosphorylated FRY; this interaction occurs in mitotic cells, but not in interphase cells (PubMed:22753416). FRY interaction facilitates AURKA-mediated PLK1 phosphorylation (PubMed:22753416). Interacts with CDK1-phosphorylated DCTN6 during mitotic prometaphase; the interaction facilitates recruitment to kinetochores (PubMed:23455152). Interacts with CEP68; the interaction phosphorylates CEP68 (PubMed:25503564). Interacts (via POLO-box domain) with DCTN1 (PubMed:20679239). Interacts with CEP20 in later G1, S, G2 and M phases of the cell cycle; this interaction recruits PLK1 to centrosomes, a step required for S phase progression (PubMed:24018379). Interacts with KLHL22 (PubMed:23455478, PubMed:24067371). Interacts (via POLO box domains) with NEDD9/HEF1 (via C-terminus) (PubMed:29191835). Interacts with FIRRM (via N-terminus region); required for maintaining, but not activating, PLK1 kinase activity (PubMed:34260926). Interacts with FZR1 (PubMed:18662541). Interacts with SKA3; the interaction promotes the stability of PLK1; the interaction promotes the stability of PLK1 (PubMed:32799774). Interacts with the MTMR3:MTMR4 heterooligomer; brings CEP55 and PLK1 together during early mitosis, regulating the phosphorylation of CEP55 by PLK1 and its recruitment to the midbody where it can mediate cell abscission (PubMed:25659891)

  • SwissProt ID

    P53350

  • Gene ID
    5347 [NCBI]
  • Synonyms

    PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13

[1]. Barr FA, et al. Polo-like kinases and the orchestration of cell division. Nat Rev Mol Cell Biol. 2004 Jun;5(6):429-40. [Content Brief]

[2]. Santamaria A, et al. The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics. 2011 Jan;10(1):M110.004457. [Content Brief]

[3]. Liu Z, et al. PLK1, A Potential Target for Cancer Therapy. Transl Oncol. 2017 Feb;10(1):22-32. [Content Brief]

[4]. de Cárcer G, et al. From Plk1 to Plk5: functional evolution of polo-like kinases. Cell Cycle. 2011 Jul 15;10(14):2255-62. [Content Brief]

[5]. Steegmaier M, et al. BI 2536, a potent and selective inhibitor of polo-like kinase 1, inhibits tumor growth in vivo. Curr Biol. 2007 Feb 20;17(4):316-22. [Content Brief]

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PLK1 Antibody (YA843) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug)using PLK1 Antibody (HY-P80972) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PLK1 with PLK1 Antibody (HY-P80972) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PLK1 Antibody (HY-P80972) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PLK1 with PLK1 Antibody (HY-P80972) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PLK1 Antibody (HY-P80972) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PLK1 Antibody (YA843) Product Image
    1/16
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • PLK1 Antibody (YA843) Product Image
  • Experimental Validation Results for PLK1 Antibody (YA843)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug)using PLK1 Antibody (HY-P80972) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PLK1 Antibody (YA843)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PLK1 with PLK1 Antibody (HY-P80972) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PLK1 Antibody (HY-P80972) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PLK1 Antibody (YA843)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PLK1 with PLK1 Antibody (HY-P80972) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PLK1 Antibody (HY-P80972) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • Experimental Validation Results for PLK1 Antibody (YA843)
  • PLK1 Antibody (YA843) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug)using PLK1 Antibody (HY-P80972) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PLK1 Antibody (YA843) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PLK1 with PLK1 Antibody (HY-P80972) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PLK1 Antibody (HY-P80972) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PLK1 Antibody (YA843) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PLK1 with PLK1 Antibody (HY-P80972) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PLK1 Antibody (HY-P80972) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PLK1 Antibody (YA843) Product Image WB
  • PLK1 Antibody (YA843) Product Image ICC
  • PLK1 Antibody (YA843) Product Image ICC