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LC3A/B Antibody

(Synonyms: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1LC3A; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B)
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Based on 1 Customer Validation

LC3A/B Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to LC3A/B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LC3A/B Antibody
  • LC3A/B Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

LC3A/B Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to LC3A/B.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 14/16 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Species Reactivity Database
Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102687

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LC3A/B Antibody
    Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg) and C2C12(lane 4(20μg) using LC3A/B (HY-P80741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Q9H492: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538)
    Q9GZQ8: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205).

  • Subcellular Localization

    Q9H492: Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor; Endomembrane system; Lipid-anchor; Cytoplasm, cytoskeleton
    Q9GZQ8: Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor; Endomembrane system; Lipid-anchor; Mitochondrion membrane; Lipid-anchor; Cytoplasm, cytoskeleton; Cytoplasmic vesicle

  • Expression


    Tissue_specificity: Q9H492: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes
    Q9GZQ8: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver

  • Isoforms & Post-Translational Modification

    Q9H492 has 2 isomers: Q9H492-1: 14272 Da (predicted); Q9H492-2: 14493 Da (predicted).
    The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I (PubMed:15187094, PubMed:30661429). The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094). During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989). ATG4 proteins also mediate the delipidation of PE-conjugated forms (PubMed:32686895, PubMed:33909989). In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989). ATG4B constitutes the major protein for proteolytic activation (PubMed:30661429, PubMed:33909989). ATG4D is the main enzyme for delipidation activity (By similarity);(Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:23112293, PubMed:31722778, PubMed:32686895). RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989);Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE)

  • Subunit

    Q9H492: Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22470510). Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy (PubMed:17580304).
    Q9GZQ8: 3 different light chains, LC1 (a cleavage product of MAP1B) , LC2 (a cleavage product of MAP1A) and LC3 (produced by one of the MAP1LC3 genes) , can associate with the MAP1A or MAP1B heavy chains (By similarity).

  • SwissProt ID

    Q9H492 / Q9GZQ8

  • Gene ID
    81631 [NCBI]
  • Synonyms

    LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1LC3A; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B

  • Research Field

    Signal Transduction

LC3A/B Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg) and C2C12(lane 4(20μg) using LC3A/B (HY-P80741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • LC3A/B Antibody Product Image
    1/16
  • Experimental Validation Results for LC3A/B Antibody
  • LC3A/B Antibody Product Image
  • Experimental Validation Results for LC3A/B Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg) and C2C12(lane 4(20μg) using LC3A/B (HY-P80741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LC3A/B Antibody
  • LC3A/B Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg) and C2C12(lane 4(20μg) using LC3A/B (HY-P80741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • LC3A/B Antibody Product Image WB